10.1371/journal.pone.0132542.g002 Maria Jalbrzikowski Maria Jalbrzikowski Maria T. Lazaro Maria T. Lazaro Fuying Gao Fuying Gao Alden Huang Alden Huang Carolyn Chow Carolyn Chow Daniel H. Geschwind Daniel H. Geschwind Giovanni Coppola Giovanni Coppola Carrie E. Bearden Carrie E. Bearden Differential expression (DE) analysis comparing controls to 22q11DS patients. Public Library of Science 2015 idiopathic psychosis expression changes antipsychotic medication use risk factors 22 q 11.2 Deletion Syndrome response pathways WGCNA analyses ht transcriptional regulation idiopathic ASD.ConclusionThese findings Functional Pathways Related brain tissue 22 q 11DS patients gene idiopathic schizophrenia 22 q 11DS peripheral blood deletion characteristics 22 q 11DS individuals pathways dysregulated 22 q 11DS carriers module Transcriptome Profiling 3 Mb deletion data mining techniques deletion breakpoints de Genomic Analyses phenotypic variation aCGH array spectrum disorders transcriptional changes 2015-07-22 03:02:56 Figure https://plos.figshare.com/articles/figure/_Differential_expression_DE_analysis_comparing_controls_to_22q11DS_patients_/1491715 <p>A) Number of DE genes at <i>p</i><0.005. Four hundred probes (245 down-regulated, 155 up-regulated) were DE in 22q11DS vs. controls. B) Gene ontology analysis (<i>q≤</i>.05) of DE genes in 22q11DS vs. controls. Top categories included ensheathment of neurons, and regulation of cellular proliferation, signal transduction, and cell communication. Bars represent the–Log of the over-representation <i>q-</i>value. Red and green represent the proportion of up and down-regulated genes, respectively. C) Heat map depicting fold changes in peripheral blood samples for each 22q11DS participant. Genes are in rows and samples are in columns. Shades of red indicate up-regulation compared to the control condition, shades of green indicate down-regulation. D) Ingenuity pathway analysis results of a top network associated with significantly DE genes (p < .005) in 22q11DS vs. controls. This network is associated with cellular development, and cellular growth and proliferation. According to IPA, 10 different molecules in this network are associated with axon guidance signaling, including genes <i>MTORC1</i>, <i>GNB1L</i> and <i>PDGFA</i>, which were all down-regulated in 22q11DS patients.</p>