%0 Figure %A Lipatova, Zhanna %A Segev, Nava %D 2015 %T Depletion of Atg9 and Atg2, but not Atg18, result in a macro-ER-phagy phenotype different from that of other core Atgs, and atg9∆ is epistatic to atg11∆. %U https://plos.figshare.com/articles/figure/_Depletion_of_Atg9_and_Atg2_but_not_Atg18_result_in_a_macro_ER_phagy_phenotype_different_from_that_of_other_core_Atgs_and_atg9_8710_is_epistatic_to_atg11_8710_/1485805 %R 10.1371/journal.pgen.1005390.g002 %2 https://ndownloader.figshare.com/files/2179012 %K erad %K ER stress %K degradation %K ERQC %K sequential steps %K core Atgs %K autophagy %K use autophagic organelles %K Ypt GTPases %K ER membrane %K upr %K ap %K system shuttles misfolded proteins %K vacuole %K role %K pathway %K ER quality control %X

A-C. While the level of overexpressed GFP-Snc1-PEM is increased in atg9∆ and atg2∆, but not atg18∆, mutant cells, it does not accumulate in aberrant structures and does not induce UPR. Wild type (WT), atg9∆, atg18∆, and atg2∆ mutant cells overexpressing GFP-Snc1-PEM were analyzed as described for Fig 1A–1C, respectively. The tested phenotypes: the level of GFP-Snc1-PEM protein (A), accumulation of GFP-Snc1-PEM in aberrant structures (B), and induction of the UPR response (C,atg1∆ is shown as a positive control). B. Shown from top to bottom: DIC, GFP, and % cells with intracellular Snc1-PEM structures. D-G. Atg9 is epistatic to Atg11 in macro-ER-phagy. ATG9 was deleted in wild type and atg11∆ mutant cells and the effects of overexpression of GFP-Snc1-PEM were determined in the single and double mutants as described in Fig 1 legend. D. Deletion of ATG9 in wild type (WT) or atg11∆ mutant cells results in an increase of GFP-Snc1-PEM protein level similar to the increase in atg11∆ mutant cells. E. Deletion of ATG9 in wild type or atg11∆ mutant cells results in an increase of intracellular GFP-Snc1-PEM fluorescence. However, only ~20% of the atg9∆ single-, and atg9∆ atg11∆ double-mutant cells accumulate GFP-Snc1-PEM in aberrant structures, as compared with ~75% of atg11∆ mutant cells. Shown from top to bottom: DIC, GFP, % cells with aberrant intracellular GFP-Snc1-PEM structures, ratio of GFP-Snc1-PEM fluorescence inside/PM (30 cells were analyzed for each strain). F. UPR is induced in atg11∆, but not in atg9∆ single- and atg9∆ atg11∆ double-mutant cells overexpressing GFP-Snc1-PEM. G. UPR can be induced in atg9∆ single-, and atg9 atg11∆ double-mutant cells overexpressing GFP-Snc1-PEM by tunicamycin. +/- and error bars represent STDEV. Results in this figure represent at least two independent experiments.

%I PLOS Genetics