TY - DATA T1 - Nitrous oxide emission potentials of Burkholderia species isolated from the leaves of a boreal peat moss Sphagnum fuscum PY - 2015/11/03 AU - Yanxia Nie AU - Li Li AU - Mengcen Wang AU - Teemu Tahvanainen AU - Yasuyuki Hashidoko UR - https://tandf.figshare.com/articles/dataset/Nitrous_oxide_emission_potentials_of_i_Burkholderia_i_species_isolated_from_the_leaves_of_a_boreal_peat_moss_i_Sphagnum_fuscum_i_a_href_FN0001_target_blank_a_/1480973 DO - 10.6084/m9.figshare.1480973.v2 L4 - https://ndownloader.figshare.com/files/2172760 L4 - https://ndownloader.figshare.com/files/2172761 KW - acetylene inhibition assay KW - N 2O reductase KW - pcr KW - N 2O emission assay KW - Nitrous oxide emission potentials KW - N 2O Burkholderia KW - N 2O emitters KW - KNO KW - N 2O Emission KW - N 2O production KW - Burkholderia sp KW - boreal peat moss Sphagnum fuscum N2 - Using a culture-based nitrous oxide (N2O) emission assay, three active N2O emitters were isolated from Sphagnum fuscum leaves and all identified as members of Burkholderia. These isolates showed N2O emission in the medium supplemented with but not with , and Burkholderia sp. SF-E2 showed the most efficient N2O emission (0.20 μg·vial−1·day−1) at 1.0 mM KNO3. In Burkholderia sp. SF-E2, the optimum pH for N2O production was 5.0, close to that of the phyllosphere of Sphagnum mosses, while the optimum temperature was uniquely over 30 °C. The stimulating effect of additional 1.5 mM sucrose on N2O emission was ignorable, but Burkholderia sp. SF-E2 upon exposure to 100 mg·L−1E-caffeic acid showed uniquely 67-fold higher N2O emission. All of the three N2O emitters were negative in both acetylene inhibition assay and PCR assay for nosZ-detection, suggesting that N2O reductase or the gene itself is missing in the N2O-emitting Burkholderia. ER -