%0 Figure %A Abu Sayeed, Md. %A Kelly Bufano, Meagan %A Xu, Peng %A Eckhoff, Grace %A C. Charles, Richelle %A Murshid Alam, Mohammad %A Sultana, Tania %A Rasheduzzaman Rashu, Md. %A Berger, Amanda %A Gonzalez-Escobedo, Geoffrey %A Mandlik, Anjali %A Rahman Bhuiyan, Taufiqur %A T. Leung, Daniel %A C. LaRocque, Regina %A B. Harris, Jason %A B. Calderwood, Stephen %A Qadri, Firdausi %A F. Vann, W. %A Kováč, Pavol %A T. Ryan, Edward %D 2015 %T (A) Immune responses against OSP linked to BSA via squarate (ratio ~5:1; OSPsq:BSA) versus an unrelated oligosaccharide linked to BSA via squarate (B; ratio ~6:1; sq:BSA-synthetic conjugate 3, see text) induced by vaccination with alum and OSP linked to rTTHc via squarate (ratio 5:1; OSP:rTTHc). %U https://plos.figshare.com/articles/figure/_A_Immune_responses_against_OSP_linked_to_BSA_via_squarate_ratio_5_1_OSPsq_BSA_versus_an_unrelated_oligosaccharide_linked_to_BSA_via_squarate_B_ratio_6_1_sq_BSA_synthetic_conjugate_3_see_text_induced_by_vaccination_with_alum_and_OSP_linked_to_rTTHc_via_sq/1477138 %R 10.1371/journal.pntd.0003881.g004 %2 https://ndownloader.figshare.com/files/2167302 %K molar loading ratio %K Memory B Cell %K lps %K cholera conjugate vaccine %K Tetanus Toxin Heavy Chain %K osp %K cholerae O 1 Inaba %K cholerae O 1 Inaba strain PIC 018 %K Mice BackgroundVibrio cholerae %K conjugate vaccine %K Intestinal lamina proprial IgA responses %K memory B cell responses %K mouse survival cholera challenge model.ConclusionWe report development %K intradermal vaccination %K immunization.Principle FindingsImmunized mice %K squaric acid chemistry %K Lamina Proprial Responses %X

Day 0 and day 56 serum from mice vaccinated with OSP:rTTHc were analyzed using plates coated with squarate-BSA (synthetic conjugate 3; see text) or OSPsq:BSA (OSP:BSA).

%I PLOS Neglected Tropical Diseases