%0 Figure %A Ramos-Molina, Bruno %A N. Lick, Adam %A Shirazi, Amir Nasrolahi %A Oh, Donghoon %A Tiwari, Rakesh %A Salem El-Sayed, Naglaa %A Parang, Keykavous %A Lindberg, Iris %D 2015 %T Cyclic polyarginine peptides inhibit cellular convertase activity. %U https://plos.figshare.com/articles/figure/_Cyclic_polyarginine_peptides_inhibit_cellular_convertase_activity_/1464677 %R 10.1371/journal.pone.0130417.g004 %2 https://ndownloader.figshare.com/files/2150211 %K cationic %K intracellular convertases %K tat %K hiv %K Chariot reagent %K furin inhibition %K HT 1080 cell migration %K micromolar inhibitors %K cyclic polyarginine peptides %K Intracellular delivery %K transfection reagents %K convertase activity %K furin activity %K proprotein convertase inhibitors %K proprotein convertases %X

(a) CHO cells were incubated with each compound at 1 μM for 24 h at 37°C, and cell viability was monitored by incubation for 4 h with WST-1. (b) CHO-GRAPfurin cells, expressing secreted alkaline phosphatase tethered to Golgi membranes by a transmembrane domain interrupted by a furin cleavage site, was incubated with 1 μM of each cyclic peptide for 20–24 h at 37 °C. Secreted alkaline phosphatase activity was measured in the medium. Results represent the mean ± S.D., N = 3.

%I PLOS ONE