TY - DATA T1 - Imaging of NTR activity in cells and in in vivo cancer model with NCL. PY - 2015/06/25 AU - Anzhelika G. Vorobyeva AU - Michael Stanton AU - Aurélien Godinat AU - Kjetil B. Lund AU - Grigory G. Karateev AU - Kevin P. Francis AU - Elizabeth Allen AU - Juri G. Gelovani AU - Emmet McCormack AU - Mark Tangney AU - Elena A. Dubikovskaya UR - https://plos.figshare.com/articles/figure/_Imaging_of_NTR_activity_in_cells_and_in_in_vivo_cancer_model_with_NCL_/1464165 DO - 10.1371/journal.pone.0131037.g005 L4 - https://ndownloader.figshare.com/files/2149531 KW - probe KW - NTR expression KW - cancer therapy approaches KW - gdept KW - Bioluminescent Nitroreductase Probe KW - ncl N2 - (A) Concentration-dependent uncaging of NCL in MDA-MB-231 NTR+luc+ cancer cells in comparison with luciferin. (B) Selectivity of NTR imaging by NCL in the same cells in comparison with NTR-luc+ cells. The dashed line indicates background (cells only), *P = 0.0001. (C) In vivo imaging of NTR activity in subcutaneous NTR+ and NTR- xenografts (n = 5). Total luminescence over 1 h from IP injection of luciferin (1.5 mg) and NCL (1.9 mg). d) Representative images of mice 15 min post injection of luciferin or NCL. ER -