%0 Figure %A G. Vorobyeva, Anzhelika %A Stanton, Michael %A Godinat, Aurélien %A B. Lund, Kjetil %A G. Karateev, Grigory %A Francis, Kevin P. %A Allen, Elizabeth %A Gelovani, Juri G. %A McCormack, Emmet %A Tangney, Mark %A Dubikovskaya, Elena A. %D 2015 %T Imaging of NTR activity in cells and in in vivo cancer model with NCL. %U https://plos.figshare.com/articles/figure/_Imaging_of_NTR_activity_in_cells_and_in_in_vivo_cancer_model_with_NCL_/1464165 %R 10.1371/journal.pone.0131037.g005 %2 https://ndownloader.figshare.com/files/2149531 %K probe %K NTR expression %K cancer therapy approaches %K gdept %K Bioluminescent Nitroreductase Probe %K ncl %X

(A) Concentration-dependent uncaging of NCL in MDA-MB-231 NTR+luc+ cancer cells in comparison with luciferin. (B) Selectivity of NTR imaging by NCL in the same cells in comparison with NTR-luc+ cells. The dashed line indicates background (cells only), *P = 0.0001. (C) In vivo imaging of NTR activity in subcutaneous NTR+ and NTR- xenografts (n = 5). Total luminescence over 1 h from IP injection of luciferin (1.5 mg) and NCL (1.9 mg). d) Representative images of mice 15 min post injection of luciferin or NCL.

%I PLOS ONE