G. Vorobyeva, Anzhelika Stanton, Michael Godinat, Aurélien B. Lund, Kjetil G. Karateev, Grigory Francis, Kevin P. Allen, Elizabeth Gelovani, Juri G. McCormack, Emmet Tangney, Mark Dubikovskaya, Elena A. <i>In vivo</i> activation of NCL probe by luciferase and nitroredictase expressing <i>E</i>.<i>coli</i> in a mouse model of thigh infection. <p>(A). Luminescence over 4 h from <i>E</i>. <i>coli luc+</i> infected quadriceps (5 × 10<sup>4</sup>–5 × 10<sup>7</sup> bacteria) after IP injection of 0.8 mg NCL probe (200 μL of 10 mM solution in PBS). (B). Luminescence over 4 h from <i>E</i>. <i>coli luc+</i> infected quadriceps (5 × 10<sup>7</sup> bacteria) following IP injection of 0.8 mg of probe or 0.63 mg of luciferin (200 μL of 10 mM solution in PBS). (C). Luminescence imaging of mice over 24 h bearing 5 × 10<sup>7</sup> bacteria, treated with various (0.08, 0.8 and 1.6 mg) NCL probe concentrations (200 μL of 1, 10 and 20 mM solutions of NCL in PBS). As a positive control, mice were injected with equal amounts of <i>E</i>. <i>coli</i> MG1655 expressing lux luciferase that doesn't require exogenous substrate for light production [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0131037#pone.0131037.ref044" target="_blank">44</a>]. The signal was collected over 24 h, n = 3 per group.</p> probe;NTR expression;cancer therapy approaches;gdept;Bioluminescent Nitroreductase Probe;ncl 2015-06-25
    https://plos.figshare.com/articles/figure/_In_vivo_activation_of_NCL_probe_by_luciferase_and_nitroredictase_expressing_E_coli_in_a_mouse_model_of_thigh_infection_/1464164
10.1371/journal.pone.0131037.g004