Light production in E. coli by NTR-mediated uncaging of NCL.
Anzhelika G. Vorobyeva
Michael Stanton
Aurélien Godinat
Kjetil B. Lund
Grigory G. Karateev
Kevin P. Francis
Elizabeth Allen
Juri G. Gelovani
Emmet McCormack
Mark Tangney
Elena A. Dubikovskaya
10.1371/journal.pone.0131037.g003
https://plos.figshare.com/articles/figure/_Light_production_in_E_coli_by_NTR_mediated_uncaging_of_NCL_/1464163
<p>(A). Light output from NCL (300 μM) after 2 h incubation in luciferase-expressing E. coli (+ luc gene) is significantly higher than in NTR mutant (NTR KO luc+) (*P < 0.001) and in wild type (- luc gene). The dashed line indicates the background. (B). Overlay of a photographic image and bioluminescence from the assay described. (C). Bioluminescence from 100 μM NCL probe and luciferin incubated with various concentrations of luciferase expressing <i>E</i>.<i>coli</i> AB1157 (10<sup>2</sup>–10<sup>8</sup> bacteria mL<sup>-1</sup>) for 10 min before imaging. (D). Bioluminescence from luciferase expressing <i>E</i>.<i>coli luc+</i> (10<sup>8</sup> bacteria mL<sup>-1</sup>) incubated with various concentrations of NCL or luciferin (1–250 μM) for 10 min before imaging.</p>
2015-06-25 03:40:59
probe
NTR expression
cancer therapy approaches
gdept
Bioluminescent Nitroreductase Probe
ncl