Light production in E. coli by NTR-mediated uncaging of NCL. Anzhelika G. Vorobyeva Michael Stanton Aurélien Godinat Kjetil B. Lund Grigory G. Karateev Kevin P. Francis Elizabeth Allen Juri G. Gelovani Emmet McCormack Mark Tangney Elena A. Dubikovskaya 10.1371/journal.pone.0131037.g003 https://plos.figshare.com/articles/figure/_Light_production_in_E_coli_by_NTR_mediated_uncaging_of_NCL_/1464163 <p>(A). Light output from NCL (300 μM) after 2 h incubation in luciferase-expressing E. coli (+ luc gene) is significantly higher than in NTR mutant (NTR KO luc+) (*P < 0.001) and in wild type (- luc gene). The dashed line indicates the background. (B). Overlay of a photographic image and bioluminescence from the assay described. (C). Bioluminescence from 100 μM NCL probe and luciferin incubated with various concentrations of luciferase expressing <i>E</i>.<i>coli</i> AB1157 (10<sup>2</sup>–10<sup>8</sup> bacteria mL<sup>-1</sup>) for 10 min before imaging. (D). Bioluminescence from luciferase expressing <i>E</i>.<i>coli luc+</i> (10<sup>8</sup> bacteria mL<sup>-1</sup>) incubated with various concentrations of NCL or luciferin (1–250 μM) for 10 min before imaging.</p> 2015-06-25 03:40:59 probe NTR expression cancer therapy approaches gdept Bioluminescent Nitroreductase Probe ncl