Chen, Jianquan Long, Fanxin Deletion of <i>Raptor</i> in preosteoblasts reduced matrix synthesis and mineralization. <p>(A-B) Alkaline phosphatase (AP) staining of wildtype (Ad-GFP)(A) versus <i>Raptor</i>-deficient (Ad-Cre)(B) calvarial cells after 7 days (D7) of osteogenic differentiation. (C-F)Von Kossastaining of wildtype (Ad-GFP)(C, E) versus <i>Raptor</i>-deficient (Ad-Cre)(D, F) calvarial cells after 14 days (D14) of osteogenic differentiation. Panels C-D showed lower magnification images; Panels E-F showed higher magnification images. (G) Quantification of bone nodules detected by Von Kossa staining. n = 3; *:P<0.05. (H) ELISA analysis of P1NP levels in wildtype (Ad-GFP) versus <i>Raptor</i>-deficient (Ad-Cre) calvarial cells after 14 days (D14) of osteogenic differentiation. n = 3; *:P<0.05.</p> osteogenic culture condition;raptor;mTORC 1 Signaling Promotes Osteoblast Differentiation;osteoblast differentiation;mTORC 1 2015-06-19
    https://plos.figshare.com/articles/figure/_Deletion_of_Raptor_in_preosteoblasts_reduced_matrix_synthesis_and_mineralization_/1455355
10.1371/journal.pone.0130627.g002