10.1371/journal.pone.0128707.g002 Tassiane Assíria Fontes Martins Tassiane Assíria Fontes Martins Lívia de Figueiredo Diniz Lívia de Figueiredo Diniz Ana Lia Mazzeti Ana Lia Mazzeti Álvaro Fernando da Silva do Nascimento Álvaro Fernando da Silva do Nascimento Sérgio Caldas Sérgio Caldas Ivo Santana Caldas Ivo Santana Caldas Isabel Mayer de Andrade Isabel Mayer de Andrade Isabela Ribeiro Isabela Ribeiro Maria Terezinha Bahia Maria Terezinha Bahia Anti-<i>Trypanosoma cruzi</i> activity of benznidazole/itraconazole combination therapy. Public Library of Science 2015 cruzi Y strain Experimental Chagas Disease combination therapy benznidazole nitroheterocyclic drugs nifurtimox Chagas disease 2015-06-15 02:54:23 Figure https://plos.figshare.com/articles/figure/_Anti_Trypanosoma_cruzi_activity_of_benznidazole_itraconazole_combination_therapy_/1449493 <p>Mice were inoculated with 5,000 <i>T</i>. <i>cruzi</i> Y strain trypomastigotes by the intraperitoneal route and treated with daily doses 50, 75 and 100 mg/kg of bodyweight (mg/kg) of benznidazole (Bz) or itraconazole (Itz) alone or in combination for 20 consecutive days. (a) Maximum number of trypomastigotes detected in the peripheral blood of treated mice up to 30 days post-treatment. (b) IgG antibodies in treated mice by ELISA up to 30 days post-treatment. The results are shown as the reactivity index value, which was obtained by dividing the absorption value (O.D. value) of each serum sample by the mean value of the differential control uninfected sample. (-) negative results in fresh blood examination and PCR assay, (+) positive results in fresh blood examination and/or PCR assay, and <b>&</b> significant difference relative to benznidazole treatment administered alone at the same dose; ★ significant relative to itraconazole treatment administered alone at the same dose.</p>