Double Nicking Nuclease Array of Gene Editing. Pawel Bialk Natalia Rivera-Torres Bryan Strouse Eric B. Kmiec 10.1371/journal.pone.0129308.g007 https://plos.figshare.com/articles/figure/_Double_Nicking_Nuclease_Array_of_Gene_Editing_/1439842 <p>Unsynchronized HCT116-19 cells were electroporated with 1ug of each of the indicated combinations of CRISPR/Cas9 nickases (1N, 2N, 3N, 4N, 5N) plus 1.35ug of 72NT. Offsets denoted with a star (*) represent nicking pairs which induce nicks on the same strand. Following electroporation, cells were allowed to incubate for 48 hours. Correction efficiency was determined by the percentage of total viable eGFP+ cells in the population as described previously. Each treatment was performed in duplicate and error bars represent standard error.</p> 2015-06-08 02:45:41 gene editing reaction strand DNA breakage Gene Editing Activity crispr ssodn DNA cleavage activity analysing reaction parameters gene editing guide RNA sequences