10.1371/journal.pone.0128039.g003 Xia Hua Xia Hua Xiaoyong Yuan Xiaoyong Yuan Zhijie Li Zhijie Li Terry G. Coursey Terry G. Coursey Stephen C. Pflugfelder Stephen C. Pflugfelder De-Quan Li De-Quan Li Dectin-1 and NF-κB signaling pathways involve PGLYRPs induction in HCECs exposed to HKCA. Public Library of Science 2015 Peptidoglycan recognition proteins Epithelial cells Candida albicans infections Human Corneal Epithelium Dectin Producing Peptidoglycan Recognition Proteins Fungal infections nf hcec tlr HKCA pglyrp Novel Innate Response role 2015-06-03 04:24:10 Figure https://plos.figshare.com/articles/figure/_Dectin_1_and_NF_954_B_signaling_pathways_involve_PGLYRPs_induction_in_HCECs_exposed_to_HKCA_/1435591 <p>A. The HCECs were exposed to 10<sup>6</sup> cells/ml HKCA with prior incubation in the absence or presence of isotype IgG (10μg/ml), dectin-1 Ab (10μg/ml), BAY11-7082 (10μM) or NF-κB activation inhibitor quinazoline (NF-κB-I, 10μM) for 1 h. Cultures treated by HKCA for 4 h were subjected to RT-qPCR to measure mRNA. B. Total protein of HCECs treated for 48 hours was extracted with RIPA buffer for western blot to examine PGLYRP-2 production. C. Protein levels of PGLYRP-2 were evaluated by western blot using β-actin as control with quantitative ratio of PGLYRP-2/β-actin. Results shown are the mean ± SD of four independent experiments; *** p<0.001, as compared with normal control; ^^ p<0.005, ^^^ p<0.001, as compared with HCECs exposed to HKCA. Magnification: 400Х (bar = 25μm).</p>