10.1371/journal.pone.0128039.g003
Xia Hua
Xia
Hua
Xiaoyong Yuan
Xiaoyong
Yuan
Zhijie Li
Zhijie
Li
Terry G. Coursey
Terry
G. Coursey
Stephen C. Pflugfelder
Stephen C.
Pflugfelder
De-Quan Li
De-Quan
Li
Dectin-1 and NF-κB signaling pathways involve PGLYRPs induction in HCECs exposed to HKCA.
Public Library of Science
2015
Peptidoglycan recognition proteins
Epithelial cells
Candida albicans infections
Human Corneal Epithelium
Dectin
Producing Peptidoglycan Recognition Proteins Fungal infections
nf
hcec
tlr
HKCA
pglyrp
Novel Innate Response
role
2015-06-03 04:24:10
Figure
https://plos.figshare.com/articles/figure/_Dectin_1_and_NF_954_B_signaling_pathways_involve_PGLYRPs_induction_in_HCECs_exposed_to_HKCA_/1435591
<p>A. The HCECs were exposed to 10<sup>6</sup> cells/ml HKCA with prior incubation in the absence or presence of isotype IgG (10μg/ml), dectin-1 Ab (10μg/ml), BAY11-7082 (10μM) or NF-κB activation inhibitor quinazoline (NF-κB-I, 10μM) for 1 h. Cultures treated by HKCA for 4 h were subjected to RT-qPCR to measure mRNA. B. Total protein of HCECs treated for 48 hours was extracted with RIPA buffer for western blot to examine PGLYRP-2 production. C. Protein levels of PGLYRP-2 were evaluated by western blot using β-actin as control with quantitative ratio of PGLYRP-2/β-actin. Results shown are the mean ± SD of four independent experiments; *** p<0.001, as compared with normal control; ^^ p<0.005, ^^^ p<0.001, as compared with HCECs exposed to HKCA. Magnification: 400Х (bar = 25μm).</p>