TY - DATA T1 - Ectopic expression of deltaD deletion and point mutant recapitulates neurogenic phenotype of DeltaD (∆A-D). PY - 2015/05/28 AU - Gregory Palardy AU - Ajay B. Chitnis UR - https://plos.figshare.com/articles/figure/_Ectopic_expression_of_deltaD_deletion_and_point_mutant_recapitulates_neurogenic_phenotype_of_DeltaD_8710_A_D_/1429266 DO - 10.1371/journal.pone.0127864.g005 L4 - https://ndownloader.figshare.com/files/2086399 KW - mid KW - bomb 1 Interaction Domain KW - mib KW - 1 Interaction Domain KW - RING E 3 ligase KW - zebrafish Notch ligand DeltaD KW - neurogenic phenotype KW - Zebrafish DeltaD Ubiquitylation N2 - (A) The prospective distribution of neurons revealed by the distribution of huC as revealed by in situ hybridization probe (purple) in control embryos injected with only ß-galactosidase mRNA. (B-F) huC in embryos co-injected with ß-galactosidase and deltaD (B), deltaD-∆C (C), deltaD ∆B (D), deltaD NN/KK (E) or DeltaD ∆A-D (F) mRNA. Distribution of ectopic mRNA injected in one cell at the two-cell stage revealed by X-Gal distribution (blue). Dorsal view, rostral to the left. Embryos are at approximately the 3 somite stage. (G) Quantification of the effect of ectopic expression of mRNA encoding various forms of DeltaD on the distribution of early neurons. Red indicates fraction with a neurogenic phenotype (increased density of neurons), Green—fraction with no obvious effect on neuron density, Blue- fraction with suppression of neurogenesis (reduced neuron density). P-values for pairwise comparison based on Fisher’s Exact test of independence. P >. 05 does not meet the criteria for the Null hypothesis that pairs contain an equivalent distribution of phenotype classes. ER -