10.1371/journal.pgen.1005265.g003 Dena H. S. Block Dena H. S. Block Kwame Twumasi-Boateng Kwame Twumasi-Boateng Hae Sung Kang Hae Sung Kang Jolie A. Carlisle Jolie A. Carlisle Alexandru Hanganu Alexandru Hanganu Ty Yu-Jen Lai Ty Yu-Jen Lai Michael Shapira Michael Shapira <i>elt-2</i> is essential for <i>atf-7</i>-dependent immune gene regulation. Public Library of Science 2015 function elegans larvae elegans GATA transcription factors SKN role atf infection resistance elt elegans immunity survival assays hydrolytic enzymes adult intestine Pseudomonas aeruginosa Infection Adult C terminal differentiation microarray analysis gene 2015-05-27 03:44:27 Figure https://plos.figshare.com/articles/figure/_elt_2_is_essential_for_atf_7_dependent_immune_gene_regulation_/1428052 <p><i>A</i>. Survival curves for wildtype and <i>atf-7(qd22qd130)</i> animals fed with designated RNAi’s during development followed by infection. Averages ± SDs for three plates (N = 129–140 per group) in a representative experiment of several others with similar results. <i>B-E</i>, Gene expression (log scale) in wildtype, <i>pmk-1(km25)</i>, and <i>atf-7(qd22qd130)</i> animals, fed with designated RNAi’s during development. Models depict for each panel the mode of disruption, or status, of examined factors (solid-line crosses, loss-of-function mutants; dashed-line crosses, knock-down), placing ELT-2 tentatively at the proximal promoter of immune genes (Gene X) putatively regulated by PMK-1 and ATF-7; <i>atf-7</i> is depicted as an activator (arrow) or repressor (blunt-ended arrow), based on disruption effects on gene expression. RNA levels were measured in L4/YA worms. Each panel presents averages and SDs for two independent experiments. Asterisks mark inter-group significance with *p<0.05, **p<0.01, and NS, non-significant (paired t-test) for all genes in the group or in underlined subset.</p>