TY - DATA T1 - Proteomic Analysis of Growth Phase-Dependent Expression of Legionella pneumophila Proteins Which Involves Regulation of Bacterial Virulence Traits PY - 2010/07/22 AU - Tsuyoshi Hayashi AU - Masahiro Nakamichi AU - Hirotaka Naitou AU - Norio Ohashi AU - Yasuyuki Imai AU - Masaki Miyake UR - https://plos.figshare.com/articles/dataset/Proteomic_Analysis_of_Growth_Phase_Dependent_Expression_of_Legionella_pneumophila_Proteins_Which_Involves_Regulation_of_Bacterial_Virulence_Traits/142547 DO - 10.1371/journal.pone.0011718 L4 - https://ndownloader.figshare.com/files/418050 L4 - https://ndownloader.figshare.com/files/418084 L4 - https://ndownloader.figshare.com/files/418123 L4 - https://ndownloader.figshare.com/files/418156 L4 - https://ndownloader.figshare.com/files/418261 KW - proteomic KW - phase-dependent KW - proteins KW - involves KW - bacterial KW - virulence KW - traits N2 - Legionella pneumophila, which is a causative pathogen of Legionnaires' disease, expresses its virulent traits in response to growth conditions. In particular, it is known to become virulent at a post-exponential phase in vitro culture. In this study, we performed a proteomic analysis of differences in expression between the exponential phase and post-exponential phase to identify candidates associated with L. pneumophila virulence using 2-Dimentional Fluorescence Difference Gel Electrophoresis (2D-DIGE) combined with Matrix-Assisted Laser Desorption/Ionization–Mass Spectrometry (MALDI-TOF-MS). Of 68 identified proteins that significantly differed in expression between the two growth phases, 64 were up-regulated at a post-exponential phase. The up-regulated proteins included enzymes related to glycolysis, ketone body biogenesis and poly-3-hydroxybutyrate (PHB) biogenesis, suggesting that L. pneumophila may utilize sugars and lipids as energy sources, when amino acids become scarce. Proteins related to motility (flagella components and twitching motility-associated proteins) were also up-regulated, predicting that they enhance infectivity of the bacteria in host cells under certain conditions. Furthermore, 9 up-regulated proteins of unknown function were found. Two of them were identified as novel bacterial factors associated with hemolysis of sheep red blood cells (SRBCs). Another 2 were found to be translocated into macrophages via the Icm/Dot type IV secretion apparatus as effector candidates in a reporter assay with Bordetella pertussis adenylate cyclase. The study will be helpful for virulent analysis of L. pneumophila from the viewpoint of physiological or metabolic modulation dependent on growth phase. ER -