TY - DATA T1 - Increased responsiveness of SP-R210L(DN) cells to LPS. PY - 2015/05/12 AU - Linlin Yang AU - Marykate Carrillo AU - Yuchieh M. Wu AU - Susan L. DiAngelo AU - Patricia Silveyra AU - Todd M. Umstead AU - E. Scott Halstead AU - Michael L. Davies AU - Sanmei Hu AU - Joanna Floros AU - Francis X. McCormack AU - Neil D. Christensen AU - Zissis C. Chroneos UR - https://plos.figshare.com/articles/figure/_Increased_responsiveness_of_SP_R210_L_DN_cells_to_LPS_/1411871 DO - 10.1371/journal.pone.0126576.g003 L4 - https://ndownloader.figshare.com/files/2064203 KW - lps KW - Myo 18A Isoforms KW - alveolar macrophages KW - expression KW - 210L KW - receptor KW - 210S KW - tlr KW - Myo 18A gene results KW - response N2 - Control and SPR210L(DN) cells were plated in 12-well tissue culture dishes at a density of 150,000 cells/well and cultured 24 hrs in RPMI/10% FBS. Cells were then treated with 100 ng/mL LPS for 2, 4, 8, 16, and 24 hrs. A) Intracellular staining of TNFα was performed in brefeldin A blocked cells 2 hrs after treatment with LPS. Dotted histograms show untreated cells. Black, red, and blue histograms show intracellular TNFα staining of control, SP-R210L(DN)1, and SP-R210L(DN)2 cells. B) The levels of secreted TNFα were measured by ELISA in media at indicated time points after treatment with LPS. (n = 6; ***p<0.005). ER -