Increased responsiveness of SP-R210<sub>L</sub>(DN) cells to LPS.
Linlin Yang
Marykate Carrillo
Yuchieh M. Wu
Susan L. DiAngelo
Patricia Silveyra
Todd M. Umstead
E. Scott Halstead
Michael L. Davies
Sanmei Hu
Joanna Floros
Francis X. McCormack
Neil D. Christensen
Zissis C. Chroneos
10.1371/journal.pone.0126576.g003
https://plos.figshare.com/articles/figure/_Increased_responsiveness_of_SP_R210_L_DN_cells_to_LPS_/1411871
<p>Control and SPR210<sub>L</sub>(DN) cells were plated in 12-well tissue culture dishes at a density of 150,000 cells/well and cultured 24 hrs in RPMI/10% FBS. Cells were then treated with 100 ng/mL LPS for 2, 4, 8, 16, and 24 hrs. A) Intracellular staining of TNFα was performed in brefeldin A blocked cells 2 hrs after treatment with LPS. Dotted histograms show untreated cells. Black, red, and blue histograms show intracellular TNFα staining of control, SP-R210<sub>L</sub>(DN)1, and SP-R210<sub>L</sub>(DN)2 cells. B) The levels of secreted TNFα were measured by ELISA in media at indicated time points after treatment with LPS. (n = 6; ***p<0.005).</p>
2015-05-12 02:54:10
lps
Myo 18A Isoforms
alveolar macrophages
expression
210L
receptor
210S
tlr
Myo 18A gene results
response