Yang, Linlin Carrillo, Marykate M. Wu, Yuchieh L. DiAngelo, Susan Silveyra, Patricia Umstead, Todd M. Scott Halstead, E. L. Davies, Michael Hu, Sanmei Floros, Joanna X. McCormack, Francis D. Christensen, Neil C. Chroneos, Zissis Dominant-negative disruption of SP-R210<sub>L</sub>. <p>Raw264.7 cells were stably transfected with empty pTriexNeo2 control or vector containing the 300 (210<sub>L</sub>(DN1)) and 350 (210<sub>L</sub>(DN2)) bp cDNA of SP-R210 carboxy-terminal isoforms (6). A) Detergent extracts were analyzed by Western blotting using affinity purified polyclonal antibodies recognizing both SP-R210<sub>L</sub> and SP-R210<sub>S</sub>. Lanes were loaded with 5 μg of protein. B) Total RNA from indicated cell lines was reverse transcribed and quantitated by qRT-PCR using TaqMan assays and primers encompassing the SP-R210<sub>L</sub>-specific exons 1 and 2 (red bars) and internal primers encompassing exon 17 and 18 common to both SP-R210 isoforms (black bars) and 18S rRNA as internal control. (n = 4 ***p<0.001).</p> lps;Myo 18A Isoforms;alveolar macrophages;expression;210L;receptor;210S;tlr;Myo 18A gene results;response 2015-05-12
    https://plos.figshare.com/articles/figure/_Dominant_negative_disruption_of_SP_R210_L_/1411869
10.1371/journal.pone.0126576.g001