10.1371/journal.pone.0126576.g001 Linlin Yang Linlin Yang Marykate Carrillo Marykate Carrillo Yuchieh M. Wu Yuchieh M. Wu Susan L. DiAngelo Susan L. DiAngelo Patricia Silveyra Patricia Silveyra Todd M. Umstead Todd M. Umstead E. Scott Halstead E. Scott Halstead Michael L. Davies Michael L. Davies Sanmei Hu Sanmei Hu Joanna Floros Joanna Floros Francis X. McCormack Francis X. McCormack Neil D. Christensen Neil D. Christensen Zissis C. Chroneos Zissis C. Chroneos Dominant-negative disruption of SP-R210<sub>L</sub>. Public Library of Science 2015 lps Myo 18A Isoforms alveolar macrophages expression 210L receptor 210S tlr Myo 18A gene results response 2015-05-12 02:54:10 Figure https://plos.figshare.com/articles/figure/_Dominant_negative_disruption_of_SP_R210_L_/1411869 <p>Raw264.7 cells were stably transfected with empty pTriexNeo2 control or vector containing the 300 (210<sub>L</sub>(DN1)) and 350 (210<sub>L</sub>(DN2)) bp cDNA of SP-R210 carboxy-terminal isoforms (6). A) Detergent extracts were analyzed by Western blotting using affinity purified polyclonal antibodies recognizing both SP-R210<sub>L</sub> and SP-R210<sub>S</sub>. Lanes were loaded with 5 μg of protein. B) Total RNA from indicated cell lines was reverse transcribed and quantitated by qRT-PCR using TaqMan assays and primers encompassing the SP-R210<sub>L</sub>-specific exons 1 and 2 (red bars) and internal primers encompassing exon 17 and 18 common to both SP-R210 isoforms (black bars) and 18S rRNA as internal control. (n = 4 ***p<0.001).</p>