10.1371/journal.pone.0126576.g001
Linlin Yang
Linlin
Yang
Marykate Carrillo
Marykate
Carrillo
Yuchieh M. Wu
Yuchieh
M. Wu
Susan L. DiAngelo
Susan
L. DiAngelo
Patricia Silveyra
Patricia
Silveyra
Todd M. Umstead
Todd M.
Umstead
E. Scott Halstead
E.
Scott Halstead
Michael L. Davies
Michael
L. Davies
Sanmei Hu
Sanmei
Hu
Joanna Floros
Joanna
Floros
Francis X. McCormack
Francis
X. McCormack
Neil D. Christensen
Neil
D. Christensen
Zissis C. Chroneos
Zissis
C. Chroneos
Dominant-negative disruption of SP-R210<sub>L</sub>.
Public Library of Science
2015
lps
Myo 18A Isoforms
alveolar macrophages
expression
210L
receptor
210S
tlr
Myo 18A gene results
response
2015-05-12 02:54:10
Figure
https://plos.figshare.com/articles/figure/_Dominant_negative_disruption_of_SP_R210_L_/1411869
<p>Raw264.7 cells were stably transfected with empty pTriexNeo2 control or vector containing the 300 (210<sub>L</sub>(DN1)) and 350 (210<sub>L</sub>(DN2)) bp cDNA of SP-R210 carboxy-terminal isoforms (6). A) Detergent extracts were analyzed by Western blotting using affinity purified polyclonal antibodies recognizing both SP-R210<sub>L</sub> and SP-R210<sub>S</sub>. Lanes were loaded with 5 μg of protein. B) Total RNA from indicated cell lines was reverse transcribed and quantitated by qRT-PCR using TaqMan assays and primers encompassing the SP-R210<sub>L</sub>-specific exons 1 and 2 (red bars) and internal primers encompassing exon 17 and 18 common to both SP-R210 isoforms (black bars) and 18S rRNA as internal control. (n = 4 ***p<0.001).</p>