TY - DATA T1 - TLR agonist treatment induced IL-10 production in microglia. PY - 2015/05/12 AU - Ayilam Ramachandran Rajalakshmy AU - Jambulingam Malathi AU - Hajib Naraharirao Madhavan UR - https://plos.figshare.com/articles/figure/_TLR_agonist_treatment_induced_IL_10_production_in_microglia_/1411842 DO - 10.1371/journal.pone.0125419.g009 L4 - https://ndownloader.figshare.com/files/2064170 KW - NS 3 KW - Tumor nicrosis factor KW - HCV NS 3 protein KW - Receptor Ligand Treatment Furnished Immune Tolerance BackgroundRecent evidence KW - TLR agonists Pam 3CSK KW - hepatitis C Virus NS 3 Mediated Microglial Inflammation KW - ligands Pam 2CSK KW - nf KW - Hepatitis C virus KW - elisa KW - Nuclear Factor kappaB KW - microglial cell line CHME 3.MethodsIL KW - NS 3 protein KW - cytokine gene expression KW - cns KW - tnf KW - NS 3. TLR ligand treatment KW - facs N2 - The microglial cells were treated with 50 ng/ml of Pam2CSK4 or Pam3CSK4 for 16 hrs. The medium was replaced with fresh growth medium with or without NS3 for another 6 hours. ELISA was performed to detect IL-10 secretion. CHME3 exposed to 20 ng/ml of NS3 for 6 hrs were used as positive control. (A) Microglial cells exposed to Pam2CSK4 or Pam3CSK4 for 16 hrs secreted IL-10 at a significant level (* p < 0.05). (B) IL-10 production was significantly up regulated in Pam2CSK+NS3 (** p < 0.01) and Pam3CSK4+NS3 (## p < 0.01) treated cells compared to NS3 alone treated cells, also IL-10 significantly up regulated in Pam3CSK4+NS3 exposed cells compared to Pam3CSK4 exposed cells (^^ p < 0.01). The data is expressed as mean (n = 3) ± SE. ER -