TY - DATA T1 - Pam3CSK4 induced immune tolerance against HCV NS3 mediated inflammation. PY - 2015/05/12 AU - Ayilam Ramachandran Rajalakshmy AU - Jambulingam Malathi AU - Hajib Naraharirao Madhavan UR - https://plos.figshare.com/articles/figure/_Pam3CSK4_induced_immune_tolerance_against_HCV_NS3_mediated_inflammation_/1411840 DO - 10.1371/journal.pone.0125419.g008 L4 - https://ndownloader.figshare.com/files/2064168 KW - NS 3 KW - Tumor nicrosis factor KW - HCV NS 3 protein KW - Receptor Ligand Treatment Furnished Immune Tolerance BackgroundRecent evidence KW - TLR agonists Pam 3CSK KW - hepatitis C Virus NS 3 Mediated Microglial Inflammation KW - ligands Pam 2CSK KW - nf KW - Hepatitis C virus KW - elisa KW - Nuclear Factor kappaB KW - microglial cell line CHME 3.MethodsIL KW - NS 3 protein KW - cytokine gene expression KW - cns KW - tnf KW - NS 3. TLR ligand treatment KW - facs N2 - Microglia was pre treated with 50 ng/ml of Pam3CSK4 for 16 hrs. RT-PCR and ELISA was performed to study the immune tolerance mediated by Pam3CSK4. (A, B, C and D) Pam3CSK4 treatment completely blocked IL-8, IL-6 and TNF-α gene expression and IL-1β gene expression was down regulated compared to positive control (PC NC represents the PCR reagent negative control. (E, F, G and H) TNF-α and IL-1β were secreted at a significant level in the Pam3CSK4 treated cells even after replacing the cells with fresh growth medium without agonist (## p< 0.01). TNF-α and IL-1β were significantly upregulated in Pam3CSK4 + NS3 treated cells compared to Pam3CSK4 exposed cells (^ p < 0.05, ^^ p < 0.01). There was a significant down regulation for IL-8 and other 3 cytokines in the Pam3CSK4 + NS3 treated cells compared to NS3 alone treated cells (** p < 0.01). The data is expressed as mean (n = 3) ± SE. ER -