10.1371/journal.pone.0125695.g001
Jie Ma
Jie
Ma
Chenying Guo
Chenying
Guo
Caiwei Guo
Caiwei
Guo
Yu Sun
Yu
Sun
Tiffany Liao
Tiffany
Liao
Ursula Beattie
Ursula
Beattie
Francisco J. López
Francisco J.
López
Dong Feng Chen
Dong
Feng Chen
Kameran Lashkari
Kameran
Lashkari
Detection of tdTomato (TD) and IGF1-TD fusion protein in human neuronal progenitor cells (hNP).
Public Library of Science
2015
iop
RGC survival
nerve fiber layers
igf
untransfected hNP cells
glaucoma
Enhances Retinal Ganglion Cell Survival
ntf
Human Neural Progenitor Cells
nerve axonal loss
td
progenitor cells
model
2015-04-29 04:35:06
Figure
https://plos.figshare.com/articles/figure/_Detection_of_tdTomato_TD_and_IGF1_TD_fusion_protein_in_human_neuronal_progenitor_cells_hNP_/1398787
<p>(A) Expression of TD protein in transfected hNPs by their red fluorescence. (B) TD protein can also be detected by using immunohistochemistry (FITC, green). (C) Expression of IGF-TD fusion protein in hNPs by their red fluorescence. (D) Detection the IGF-1 moiety of the IGF-TD fusion protein in transfected cells by immunostaining (FITC, green). (E) Expression of IGF-1 component of the IGF-TD mRNA in hNP<sup>IGF-TD</sup> cells by qRT-PCR; IGF-1 mRNA is undetectable in untransfected hNP and hNP<sup>TD</sup> cells. (F) Western blot analysis of IGF-1 component of the IGF-TD protein in hNP<sup>IGF-TD</sup> cell lysates confirms the expected molecular weight of approximately 60 kD. IGF-1 was not detected in untransfected hNP and hNP<sup>TD</sup> cells. (G) IGF-1 levels are higher in the medium of cells transfected with the IGF-TD fusion protein (red triangles). Data are presented as mean ± SE for the day 0 wells (B; blank wells). Symbols correspond to the fitted means ± the 95% confidence limits of the measurements conducted in conditioned medium from the difference hNPs (open symbols; empty vector, yellow symbols; TD-transfected cells, green symbols; IGF-TD—transfected cells). Analysis was conducted by fitting a two way linear model for treatments and time points on the inverse of the data. Multiple comparisons were conducted on the transformed scale by the Tukey test. Asterisks indicate statistically significant differences <i>versus</i> all groups at the same time point. Abbreviations: hNP, neuronal progenitor cells; TD, tdTomato; IGF-TD, IGF-1-tdTomato. Scale bar in A—D: 50 μm.</p>