10.6084/m9.figshare.1397443.v2
Dong-Gi Lee
Dong-Gi
Lee
Juhyun Nam
Juhyun
Nam
Sam Woong Kim
Sam
Woong Kim
Young-Moon Kang
Young-Moon
Kang
Hyun Joo An
Hyun Joo
An
Chul Wook Kim
Chul
Wook Kim
Jong-Soon Choi
Jong-Soon
Choi
Proteomic analysis of reproduction proteins involved in litter size from porcine placenta
Taylor & Francis Group
2015
LLSG
protein
litter size group
SLSG
increase litter size
rbp
porcine placenta
psa
analysis
porcine fertility traits
2015-08-04 08:01:16
Dataset
https://tandf.figshare.com/articles/dataset/Proteomic_analysis_of_reproduction_proteins_involved_in_litter_size_from_porcine_placenta/1397443
<div><p>A gel-free and label-free quantitative proteomic approach based on a spectral counting strategy was performed to discover prolificacy-related proteins. Soluble proteins of porcine placenta from small litter size group (SLSG) and large litter size group (LLSG) were extracted and subsequently applied to in-solution tryptic digestion followed by liquid chromatography–tandem mass spectrometry analysis. Six and thirteen proteins were highly expressed in SLSG and LLSG, respectively. Of the dominantly expressed proteins, we chose prolificacy-related proteins such as puromycin-sensitive aminopeptidase (PSA) and retinol-binding protein 4 (RBP4). Western blot analysis confirmed that the processed form (70 kDa) of PSA was more expressed and RBP4 (23 kDa) was dominantly expressed in LLSG. These results indicate that PSA and RBP4 are representative proteins involved in porcine fertility traits, and this finding may help to increase litter size of pigs.</p></div>