10.6084/m9.figshare.1397443.v2 Dong-Gi Lee Dong-Gi Lee Juhyun Nam Juhyun Nam Sam Woong Kim Sam Woong Kim Young-Moon Kang Young-Moon Kang Hyun Joo An Hyun Joo An Chul Wook Kim Chul Wook Kim Jong-Soon Choi Jong-Soon Choi Proteomic analysis of reproduction proteins involved in litter size from porcine placenta Taylor & Francis Group 2015 LLSG protein litter size group SLSG increase litter size rbp porcine placenta psa analysis porcine fertility traits 2015-08-04 08:01:16 Dataset https://tandf.figshare.com/articles/dataset/Proteomic_analysis_of_reproduction_proteins_involved_in_litter_size_from_porcine_placenta/1397443 <div><p>A gel-free and label-free quantitative proteomic approach based on a spectral counting strategy was performed to discover prolificacy-related proteins. Soluble proteins of porcine placenta from small litter size group (SLSG) and large litter size group (LLSG) were extracted and subsequently applied to in-solution tryptic digestion followed by liquid chromatography–tandem mass spectrometry analysis. Six and thirteen proteins were highly expressed in SLSG and LLSG, respectively. Of the dominantly expressed proteins, we chose prolificacy-related proteins such as puromycin-sensitive aminopeptidase (PSA) and retinol-binding protein 4 (RBP4). Western blot analysis confirmed that the processed form (70 kDa) of PSA was more expressed and RBP4 (23 kDa) was dominantly expressed in LLSG. These results indicate that PSA and RBP4 are representative proteins involved in porcine fertility traits, and this finding may help to increase litter size of pigs.</p></div>