TY - DATA T1 - Mongolian Almond (Prunus mongolica Maxim): The Morpho-Physiological, Biochemical and Transcriptomic Response to Drought Stress PY - 2015/04/20 AU - JĒ–gang Wang AU - Rong Zheng AU - Shulan Bai AU - Xiaomin Gao AU - Min Liu AU - Wei Yan UR - https://plos.figshare.com/articles/dataset/Mongolian_Almond_Prunus_mongolica_Maxim_The_Morpho_Physiological_Biochemical_and_Transcriptomic_Response_to_Drought_Stress/1385873 DO - 10.1371/journal.pone.0124442 L4 - https://ndownloader.figshare.com/files/2029306 L4 - https://ndownloader.figshare.com/files/2029307 L4 - https://ndownloader.figshare.com/files/2029308 L4 - https://ndownloader.figshare.com/files/2029309 L4 - https://ndownloader.figshare.com/files/2029310 L4 - https://ndownloader.figshare.com/files/2029311 L4 - https://ndownloader.figshare.com/files/2029312 L4 - https://ndownloader.figshare.com/files/2029313 KW - mongolica seedlings KW - Drought Stress Prunus mongolica Maxim KW - Gobi Desert KW - iron superoxide dismutase transcription KW - drought stress KW - drought response genes KW - superoxide dismutase gene families KW - manganese superoxide dismutase KW - protein transcripts N2 - Prunus mongolica Maxim, which is widely established in the Gobi Desert, shows extreme tolerance to drought. However, there is a lack of available transcriptomic resources for this species related to its response to water deficiency. To investigate the mechanisms that allow P. mongolica to maintain growth in extremely arid environments, the response of P. mongolica seedlings to drought stress was analyzed using morphological, physiological, biochemical and high-throughput sequencing approaches. We generated 28,713,735 and 26,650,133 raw reads from no-stress control and drought-stressed P. mongolica seedlings, respectively. In total, we obtained 67,352 transcripts with an average length of 874.44 bp. Compared with the no-stress control, 3,365 transcripts were differentially expressed in the drought-stressed seedlings, including 55.75% (1,876 transcripts) up-regulated and 44.25% (1,489 transcripts) down-regulated transcripts. The photosynthesis response showed a decreasing tendency under drought stress, but the changes in the levels of hormones (auxins, cytokinins and abscisic acid) resulted in the closing of stomata and decreased cell enlargement and division; these changes were effective for promoting P. mongolica survival in Gobi Desert. Next, we analyzed the aquaporin and superoxide dismutase gene families due to their importance in plant resistance to drought stress. We found that all of the plasma membrane intrinsic protein transcripts were down-regulated in the drought-stressed treatment, whereas drought did not affect the expression of nodulin intrinsic protein or small basic intrinsic protein transcripts in P. mongolica seedlings. In addition, activation of iron superoxide dismutase transcription and enhanced transcription of manganese superoxide dismutase were observed in P. mongolica to promote tolerance of drought stress. This study identified drought response genes in P. mongolica seedlings. Our results provide a significant contribution to the understanding of how P. mongolica responds to drought stress at the transcriptome level, which may help to elucidate molecular mechanisms associated with the drought response of almond plants. ER -