TY - DATA T1 - SMAD signaling and redox imbalance cooperate to induce prostate cancer cell dormancy PY - 2015/10/12 AU - Anh Thu Bui AU - Fanny Laurent AU - Maryline Havard AU - François Dautry AU - Thierry Tchénio UR - https://tandf.figshare.com/articles/dataset/SMAD_signaling_and_redox_imbalance_cooperate_to_induce_prostate_cancer_cell_dormancy/1384855 DO - 10.6084/m9.figshare.1384855.v5 L4 - https://ndownloader.figshare.com/files/2028132 L4 - https://ndownloader.figshare.com/files/2028137 L4 - https://ndownloader.figshare.com/files/2028133 L4 - https://ndownloader.figshare.com/files/2028134 L4 - https://ndownloader.figshare.com/files/2028135 L4 - https://ndownloader.figshare.com/files/2356214 KW - cell proliferation KW - cell signaling KW - prostate cancer KW - redox regulation KW - SMAD transcription factor N2 - Metastasis involves the dissemination of single or small clumps of cancer cells through blood or lymphatic vessels and their extravasation into distant organs. Despite the strong regulation of metastases development by a cell dormancy phenomenon, the dormant state of cancer cells remains poorly characterized due to the difficulty of in vivo studies. We have recently shown in vitro that clonogenicity of prostate cancer cells is regulated by a dormancy phenomenon that is strongly induced when cells are cultured both at low cell density and in a slightly hypertonic medium. Here, we characterized by RT-qPCR a genetic expression signature of this dormant state which combines the presence of both stemness and differentiation markers. We showed that both TFGβ/BMP signaling and redox imbalance are required for the full induction of this dormancy signature and cell quiescence. Moreover, reconstruction experiments showed that TFGβ/BMP signaling and redox imbalance are sufficient to generate a pattern of genetic expression displaying all characteristic features of the dormancy signature. Finally, we observed that low cell density was sufficient to activate TGFβ/BMP signaling and to generate a slight redox imbalance thus priming cells for dormancy that can be attained with a co-stimulus like hypertonicity, most likely through an increased redox imbalance. The identification of a dual regulation of dormancy provides a framework for the interpretation of previous reports showing a restricted ability of BMP signaling to regulate cancer cell dormancy in vivo and draws attention on the role of oxidative stress in the metastatic process. ER -