10.1371/journal.pone.0123724.g008 Dengfeng Gao Dengfeng Gao Guanghua Hao Guanghua Hao Zhe Meng Zhe Meng Ning Ning Ning Ning Guang Yang Guang Yang Zhongwei Liu Zhongwei Liu Xin Dong Xin Dong Xiaolin Niu Xiaolin Niu ERK signaling pathway in the effects of PPAR-γ agonist on Ang II-mediated KLF5 expression. Public Library of Science 2015 growth response protein Rat Vascular Smooth Muscle Cells Kr üppel factor target gene cyclin D 1 gw pkc protein kinase C KLF 5 expression VSMC proliferation rosiglitazone ang ppar Cyclin D 1. KLF 5 erk mechanism ii 2015-04-14 02:49:11 Figure https://plos.figshare.com/articles/figure/_ERK_signaling_pathway_in_the_effects_of_PPAR_947_agonist_on_Ang_II_mediated_KLF5_expression_/1379148 <p>(A) VSMCs were subjected to ERK1/2 inhibitor PD098059 (1 μM) for 30min, followed by treatment of rosiglitazone (5 μM) for further 1 h, and finally stimulated with Ang II (0.1 mM) for 24 h. A representative western blot of KLF5 (upper panel), and data of mean ± S.E.M. of 3 experiments (bottom panel) were shown. (B) VSMCs were pretreated with rosiglitazone (5 μM) for 1h, and incubated with Ang II (0.1 μM) for 30 min, phosphorylated ERK1/2 were then detected by western blot. A representative western blot of ERK1/2 and p-ERK1/2, and data of mean ± S.E.M. of 3 experiments (bottom panel) were shown. Results are expressed as fold increase over control group. β-actin served as an internal control. (*<i>P<0</i>.<i>05</i> vs. control; #<i>P<0</i>.<i>05</i> vs. Ang II.)</p>