10.1371/journal.pone.0123724.g008
Dengfeng Gao
Dengfeng
Gao
Guanghua Hao
Guanghua
Hao
Zhe Meng
Zhe
Meng
Ning Ning
Ning
Ning
Guang Yang
Guang
Yang
Zhongwei Liu
Zhongwei
Liu
Xin Dong
Xin
Dong
Xiaolin Niu
Xiaolin
Niu
ERK signaling pathway in the effects of PPAR-γ agonist on Ang II-mediated KLF5 expression.
Public Library of Science
2015
growth response protein
Rat Vascular Smooth Muscle Cells Kr üppel factor
target gene cyclin D 1
gw
pkc
protein kinase C
KLF 5 expression
VSMC proliferation
rosiglitazone
ang
ppar
Cyclin D 1.
KLF 5
erk
mechanism
ii
2015-04-14 02:49:11
Figure
https://plos.figshare.com/articles/figure/_ERK_signaling_pathway_in_the_effects_of_PPAR_947_agonist_on_Ang_II_mediated_KLF5_expression_/1379148
<p>(A) VSMCs were subjected to ERK1/2 inhibitor PD098059 (1 μM) for 30min, followed by treatment of rosiglitazone (5 μM) for further 1 h, and finally stimulated with Ang II (0.1 mM) for 24 h. A representative western blot of KLF5 (upper panel), and data of mean ± S.E.M. of 3 experiments (bottom panel) were shown. (B) VSMCs were pretreated with rosiglitazone (5 μM) for 1h, and incubated with Ang II (0.1 μM) for 30 min, phosphorylated ERK1/2 were then detected by western blot. A representative western blot of ERK1/2 and p-ERK1/2, and data of mean ± S.E.M. of 3 experiments (bottom panel) were shown. Results are expressed as fold increase over control group. β-actin served as an internal control. (*<i>P<0</i>.<i>05</i> vs. control; #<i>P<0</i>.<i>05</i> vs. Ang II.)</p>