PKC signaling pathway in the effect of PPAR-γ agonist on Ang II-induced KLF5 expression. Dengfeng Gao Guanghua Hao Zhe Meng Ning Ning Guang Yang Zhongwei Liu Xin Dong Xiaolin Niu 10.1371/journal.pone.0123724.g007 https://plos.figshare.com/articles/figure/_PKC_signaling_pathway_in_the_effect_of_PPAR_947_agonist_on_Ang_II_induced_KLF5_expression_/1379145 <p>(A) and (B). VSMCs were pretreated with rosiglitazone (5 μM) for 1hr, and incubated with Ang II (0.1 μM) for 30 min; phosphorylated PKCε and PKCζ were then detected by western blot. Representative western blot of p-PKCε and p-PKCζ, and data of mean ± S.E.M. of 3 experiments (bottom panel) were shown. (C). VSMCs were pretreated with PKCζ siRNA for 48 hrs prior to the addition of rosiglitazone (5 μM) for 1 h, and subsequently stimulated with Ang II (0.1 μM) for 24 h. A representative western blot of KLF5, and data of mean ± S.E.M. of 3 experiments (bottom panel) were shown. All results are expressed as fold increase over control group. β-actin served as an internal control. (*<i>P<0</i>.<i>05</i> vs. control; #<i>P<0</i>.<i>05</i> vs. Ang II).</p> 2015-04-14 02:49:11 growth response protein Rat Vascular Smooth Muscle Cells Kr üppel factor target gene cyclin D 1 gw pkc protein kinase C KLF 5 expression VSMC proliferation rosiglitazone ang ppar Cyclin D 1. KLF 5 erk mechanism ii