10.1371/journal.pgen.1005092.g007
Aymeric Chartier
Aymeric
Chartier
Pierre Klein
Pierre
Klein
Stéphanie Pierson
Stéphanie
Pierson
Nicolas Barbezier
Nicolas
Barbezier
Teresa Gidaro
Teresa
Gidaro
François Casas
François
Casas
Steven Carberry
Steven
Carberry
Paul Dowling
Paul
Dowling
Laurie Maynadier
Laurie
Maynadier
Maëlle Bellec
Maëlle
Bellec
Martine Oloko
Martine
Oloko
Claude Jardel
Claude
Jardel
Bodo Moritz
Bodo
Moritz
George Dickson
George
Dickson
Vincent Mouly
Vincent
Mouly
Kay Ohlendieck
Kay
Ohlendieck
Gillian Butler-Browne
Gillian
Butler-Browne
Capucine Trollet
Capucine
Trollet
Martine Simonelig
Martine
Simonelig
Deregulation of the mitochondrial pathway in the OPMD mouse model.
Public Library of Science
2015
ccr
mRNAs encoding mitochondrial proteins
cleavage
poly
Drosophila OPMD model uncovers
deadenylation regulator Smaug
pabpn
candidate genes encoding RNA binding proteins
role
Oculopharyngeal Muscular Dystrophy Pathogenesis Oculopharyngeal
mitochondrial dysfunction
2015-03-27 04:22:30
Figure
https://plos.figshare.com/articles/figure/_Deregulation_of_the_mitochondrial_pathway_in_the_OPMD_mouse_model_/1359255
<p>A) Number of deregulated genes in A17.1 mouse skeletal muscles using microarrays. T1, 6 weeks; T2, 18 weeks; T3, 26 weeks. B) Venn diagram of overlapping down-regulated genes at T1, T2 and T3. C) Number of nuclear genes encoding mitochondrial respiratory chain complex subunits down-regulated in A17.1 mouse muscles. D) Quantification of levels of mRNAs encoding mitochondrial respiratory chain subunits in control (WT) and A17.1 quadriceps skeletal muscle at T1, using RT-qPCR. Normalization was with <i>Rplp0</i> mRNA. Means are from three biological replicates, error bars represent standard deviation. * <i>p</i>-value <0.05, ** <i>p</i>-value <0.01, *** <i>p</i>-value <0.001, using the Student’s t-Test. E) ePAT assays of mRNAs encoding mitochondrial proteins in control (WT) and A17.1 quadriceps skeletal muscles. Arrows indicate poly(A) tails of 12A. Accumulation of 12A poly(A) tails was visible in A17.1 muscles at T2 and/or T3. <i>RpL32</i> is a negative control mRNA encoding a ribosomal protein. Profiles of ePAT assays using the ImageJ software are shown.</p>