Chartier, Aymeric Klein, Pierre Pierson, Stéphanie Barbezier, Nicolas Gidaro, Teresa Casas, François Carberry, Steven Dowling, Paul Maynadier, Laurie Bellec, Maëlle Oloko, Martine Jardel, Claude Moritz, Bodo Dickson, George Mouly, Vincent Ohlendieck, Kay Butler-Browne, Gillian Trollet, Capucine Simonelig, Martine Defective cleavage at poly(A) sites in muscles expressing PABPN1-17ala and in the <i>Pabp2</i> mutant. <p>A) Schematic representation of primers (arrows) used to quantify uncleaved pre-mRNA. B) Quantification of uncleaved pre-mRNAs in control and PABPN1-17ala-expressing thoraxes at day 2, using RT-qPCR. Uncleaved RNAs were normalized to <i>Cpr100A</i> uncleaved RNA. <i>Cpr100A</i> is expressed in the cuticle and its expression remains unaffected by expression of PABPN1-17ala in muscles. Means of three biological replicates quantified three times. For (B) and (C), error bars represent standard deviation. * <i>p</i>-value <0.05, ** <i>p</i>-value <0.01, *** <i>p</i>-value <0.001, ns: not significant, using the Student’s t-Test. C) Quantification of uncleaved pre-mRNAs in control (<i>w</i><sup><i>1118</i></sup>) and <i>Pabp2</i> (<i>Pabp2</i><sup><i>55</i></sup><i>/Df(2R)CA53</i>) mutant first instar larvae, using RT-qPCR. Uncleaved RNAs were normalized to <i>RpS6</i> uncleaved RNA. The levels of <i>RpS6</i> uncleaved RNA normalized to <i>sop</i> mRNA were unaffected in <i>Pabp2</i> mutant larvae (right panel). Means of three biological replicates quantified three times.</p> ccr;mRNAs encoding mitochondrial proteins;cleavage;poly;Drosophila OPMD model uncovers;deadenylation regulator Smaug;pabpn;candidate genes encoding RNA binding proteins;role;Oculopharyngeal Muscular Dystrophy Pathogenesis Oculopharyngeal;mitochondrial dysfunction 2015-03-27
    https://plos.figshare.com/articles/figure/_Defective_cleavage_at_poly_A_sites_in_muscles_expressing_PABPN1_17ala_and_in_the_Pabp2_mutant_/1359252
10.1371/journal.pgen.1005092.g004