10.1371/journal.pone.0121574.g004 Xueqi Qu Xueqi Qu Maren Pröll Maren Pröll Christiane Neuhoff Christiane Neuhoff Rui Zhang Rui Zhang Mehmet Ulas Cinar Mehmet Ulas Cinar Md. Munir Hossain Md. Munir Hossain Dawit Tesfaye Dawit Tesfaye Christine Große-Brinkhaus Christine Große-Brinkhaus Dessie Salilew-Wondim Dessie Salilew-Wondim Ernst Tholen Ernst Tholen Christian Looft Christian Looft Michael Hölker Michael Hölker Karl Schellander Karl Schellander Muhammad Jasim Uddin Muhammad Jasim Uddin SFN inhibits HDAC activity and regulates genes which encode epigenetic enzymes. Public Library of Science 2015 HDAC activity HDAC inhibitor Lipopolysaccharide Histone acetylation lipopolysaccharide LPS Sulforaphane Epigenetically Regulates Innate Immune Responses IRF 6 gene expressions DNMT 3a gene expression LPS stimulation DNMT 1 gene tbp il porcine moDCs HDAC inhibitor SFN nf CD 86 expression tlr moDCs differentiation tgf cell culture supernatants cell apoptosis histone deacetylases DNA methyltransferase tnf MD 2 gene expression response HDAC family gene expressions Dendritic cells 2015-03-20 04:04:55 Figure https://plos.figshare.com/articles/figure/_SFN_inhibits_HDAC_activity_and_regulates_genes_which_encode_epigenetic_enzymes_/1345726 <p>moDCs at day 7 in cell culture were used for this experiment. Relative HDAC activity assay was measured using the Color-de-Lys HDAC colorimetric activity assay kit. To confirm the global HDAC deacetylation of moDCs, cells were stimulated with different concentration of SFN (Control, 5 μM, 10 μM, 15 μM, and 20 μM) for 24 h (A). Equal amounts of isolated nuclear protein were subjected to HDAC activity analysis. The effects of SFN (10 μM) on gene expression of epigenetic encoding enzymes in porcine moDCs stimulated with LPS were examined. The HDAC6 (B), HDAC10 (C), DNMT3a (D) and DNMT1 (E) mRNA expression was quantified using qRT-PCR. The moDCs were pre-treated for 24 h with or without SFN before stimulating with LPS (1 μg/ml) for additional 24 h. The results (A, B, C, D, and E) were represented as the mean ± standard deviation (SD) of three independent experiments and each experiment was performed in duplicate (*p < 0.05; **p < 0.01; ***p < 0.001).</p>