10.1371/journal.pone.0121574.g004
Xueqi Qu
Xueqi
Qu
Maren Pröll
Maren
Pröll
Christiane Neuhoff
Christiane
Neuhoff
Rui Zhang
Rui
Zhang
Mehmet Ulas Cinar
Mehmet
Ulas Cinar
Md. Munir Hossain
Md.
Munir Hossain
Dawit Tesfaye
Dawit
Tesfaye
Christine Große-Brinkhaus
Christine
Große-Brinkhaus
Dessie Salilew-Wondim
Dessie
Salilew-Wondim
Ernst Tholen
Ernst
Tholen
Christian Looft
Christian
Looft
Michael Hölker
Michael
Hölker
Karl Schellander
Karl
Schellander
Muhammad Jasim Uddin
Muhammad
Jasim Uddin
SFN inhibits HDAC activity and regulates genes which encode epigenetic enzymes.
Public Library of Science
2015
HDAC activity
HDAC inhibitor
Lipopolysaccharide Histone acetylation
lipopolysaccharide LPS
Sulforaphane Epigenetically Regulates Innate Immune Responses
IRF 6
gene expressions
DNMT 3a gene expression
LPS stimulation
DNMT 1 gene
tbp
il
porcine moDCs
HDAC inhibitor SFN
nf
CD 86 expression
tlr
moDCs differentiation
tgf
cell culture supernatants
cell apoptosis
histone deacetylases
DNA methyltransferase
tnf
MD 2 gene expression
response
HDAC family gene expressions
Dendritic cells
2015-03-20 04:04:55
Figure
https://plos.figshare.com/articles/figure/_SFN_inhibits_HDAC_activity_and_regulates_genes_which_encode_epigenetic_enzymes_/1345726
<p>moDCs at day 7 in cell culture were used for this experiment. Relative HDAC activity assay was measured using the Color-de-Lys HDAC colorimetric activity assay kit. To confirm the global HDAC deacetylation of moDCs, cells were stimulated with different concentration of SFN (Control, 5 μM, 10 μM, 15 μM, and 20 μM) for 24 h (A). Equal amounts of isolated nuclear protein were subjected to HDAC activity analysis. The effects of SFN (10 μM) on gene expression of epigenetic encoding enzymes in porcine moDCs stimulated with LPS were examined. The HDAC6 (B), HDAC10 (C), DNMT3a (D) and DNMT1 (E) mRNA expression was quantified using qRT-PCR. The moDCs were pre-treated for 24 h with or without SFN before stimulating with LPS (1 μg/ml) for additional 24 h. The results (A, B, C, D, and E) were represented as the mean ± standard deviation (SD) of three independent experiments and each experiment was performed in duplicate (*p < 0.05; **p < 0.01; ***p < 0.001).</p>