TY - DATA T1 - The bona fide DNA-binding domain of Sp2 is dispensable for genomic binding. PY - 2015/03/20 AU - Sara Völkel AU - Bastian Stielow AU - Florian Finkernagel AU - Thorsten Stiewe AU - Andrea Nist AU - Guntram Suske UR - https://plos.figshare.com/articles/figure/_The_bona_fide_DNA_binding_domain_of_Sp2_is_dispensable_for_genomic_binding_/1345722 DO - 10.1371/journal.pgen.1005102.g004 L4 - https://ndownloader.figshare.com/files/1964595 KW - genomic binding pattern KW - genomic binding site selection KW - ccaat KW - gc KW - Sp 3 mutants KW - Sp 3 Transcription factors KW - genomic binding patterns KW - Sp 3 KW - Sp 2 KW - binding site selection KW - mef KW - Sp 2 potentiates binding KW - Sp 2 Potentiates Recruitment N2 - Sp2ko MEFs re-expressing Flag-tagged full-length Sp2 (Flag-Sp2FL), the N-terminal domain (Flag-Sp2NT) or the C-terminal zinc finger domains (Flag-Sp2ZF) were subjected to ChIP-seq analysis. (A) Venn diagram showing the overlap of sites bound by Flag-Sp2FL, Flag-Sp2NT and Flag-Sp2ZF with sites bound by endogenous Sp2 in wild type MEFs. (B) Full-length Sp2 and the N-terminal region of Sp2 have similar chromatin binding efficiencies. ChIP-seq tag counts (normalized to 20x106 reads) at individual Flag-Sp2NT and Flag-Sp2FL peaks found in both samples were plotted against each other. (C) Sites bound by Flag-Sp2NT represent high affinity binding sites of native Sp2. Individual native Sp2 peaks [18] were plotted against their normalized tag counts. Those sites that were also detected by ChIP-seq in Sp2ko MEFs expressing the N-terminal domain of Sp2 (Flag-Sp2NT mutant) were overlaid with red dots. (D) Representative binding profiles of Sp2 in wild type MEFs (Sp2 / wt), and of Flag-Sp2FL, Flag-Sp2NT and Flag-Sp2ZF expressed in Sp2ko MEFs. (E) Schematic representation of the genomic binding features of Sp1/Sp3 and Sp2 based on the results shown in Figs. 1–4. ER -