The Luciferase Immunoprecipitation Systems (LIPS).
Shunya Nakane
Osamu Higuchi
Michiaki Koga
Takashi Kanda
Kenya Murata
Takashi Suzuki
Hiroko Kurono
Masanari Kunimoto
Ken-ichi Kaida
Akihiro Mukaino
Waka Sakai
Yasuhiro Maeda
Hidenori Matsuo
10.1371/journal.pone.0118312.g002
https://plos.figshare.com/articles/figure/_The_Luciferase_Immunoprecipitation_Systems_LIPS_/1343464
<p>The soluble fractionated component from the solubilized HEK 293F cells, including the gAChRα3 or β4-GL, reacted with human serum, and the specific luciferase activities of the gAChRα3 or β4-GL were found with the luminometer. The <i>in vitro</i> LIPS assay can quantitatively evaluate an interaction between an antigen and an antibody with high sensitivity and without a radioisotope.</p>
2015-03-19 03:35:42
luciferase immunoprecipitation systems
rip
Ganglionic Acetylcholine Receptor
lips
gAChR
seronegative group
Autoantibodie
aag
Japanese Patients Autoimmune
ganglionic nicotinic acetylcholine receptors
seropositive group
Autoimmune Autonomic Ganglionopathy