The Luciferase Immunoprecipitation Systems (LIPS). Shunya Nakane Osamu Higuchi Michiaki Koga Takashi Kanda Kenya Murata Takashi Suzuki Hiroko Kurono Masanari Kunimoto Ken-ichi Kaida Akihiro Mukaino Waka Sakai Yasuhiro Maeda Hidenori Matsuo 10.1371/journal.pone.0118312.g002 https://plos.figshare.com/articles/figure/_The_Luciferase_Immunoprecipitation_Systems_LIPS_/1343464 <p>The soluble fractionated component from the solubilized HEK 293F cells, including the gAChRα3 or β4-GL, reacted with human serum, and the specific luciferase activities of the gAChRα3 or β4-GL were found with the luminometer. The <i>in vitro</i> LIPS assay can quantitatively evaluate an interaction between an antigen and an antibody with high sensitivity and without a radioisotope.</p> 2015-03-19 03:35:42 luciferase immunoprecipitation systems rip Ganglionic Acetylcholine Receptor lips gAChR seronegative group Autoantibodie aag Japanese Patients Autoimmune ganglionic nicotinic acetylcholine receptors seropositive group Autoimmune Autonomic Ganglionopathy