10.1371/journal.pone.0024585
Brent L. Williams
Brent
L. Williams
Mady Hornig
Mady
Hornig
Timothy Buie
Timothy
Buie
Margaret L. Bauman
Margaret
L. Bauman
Myunghee Cho Paik
Myunghee
Cho Paik
Ivan Wick
Ivan
Wick
Ashlee Bennett
Ashlee
Bennett
Omar Jabado
Omar
Jabado
David L. Hirschberg
David
L. Hirschberg
W. Ian Lipkin
W. Ian
Lipkin
Impaired Carbohydrate Digestion and Transport and Mucosal Dysbiosis in the Intestines of Children with Autism and Gastrointestinal Disturbances
Public Library of Science
2011
impaired
digestion
mucosal
dysbiosis
intestines
children
autism
gastrointestinal
disturbances
2011-09-16 00:59:14
Dataset
https://plos.figshare.com/articles/dataset/Impaired_Carbohydrate_Digestion_and_Transport_and_Mucosal_Dysbiosis_in_the_Intestines_of_Children_with_Autism_and_Gastrointestinal_Disturbances/133554
<div><p>Gastrointestinal disturbances are commonly reported in children with autism, complicate clinical management, and may contribute to behavioral impairment. Reports of deficiencies in disaccharidase enzymatic activity and of beneficial responses to probiotic and dietary therapies led us to survey gene expression and the mucoepithelial microbiota in intestinal biopsies from children with autism and gastrointestinal disease and children with gastrointestinal disease alone. Ileal transcripts encoding disaccharidases and hexose transporters were deficient in children with autism, indicating impairment of the primary pathway for carbohydrate digestion and transport in enterocytes. Deficient expression of these enzymes and transporters was associated with expression of the intestinal transcription factor, CDX2. Metagenomic analysis of intestinal bacteria revealed compositional dysbiosis manifest as decreases in Bacteroidetes, increases in the ratio of Firmicutes to Bacteroidetes, and increases in Betaproteobacteria. Expression levels of disaccharidases and transporters were associated with the abundance of affected bacterial phylotypes. These results indicate a relationship between human intestinal gene expression and bacterial community structure and may provide insights into the pathophysiology of gastrointestinal disturbances in children with autism.</p> </div>