10.1371/journal.pone.0114830.g004 I-Chun Lai I-Chun Lai Ping-Hsiao Shih Ping-Hsiao Shih Chih-Jung Yao Chih-Jung Yao Chi-Tai Yeh Chi-Tai Yeh Jacqueline Wang-Peng Jacqueline Wang-Peng Tai-Ngar Lui Tai-Ngar Lui Suang-En Chuang Suang-En Chuang Tsai-Shu Hu Tsai-Shu Hu Tung-Yuan Lai Tung-Yuan Lai Gi-Ming Lai Gi-Ming Lai The expression of stemness genes in GBM8401 parental, SP and non-SP (NSP) cells. Public Library of Science 2015 mgmt Notch 3 honokiol SP cells ihh GBM 8401 SP cells csc tmz smo GBM 8401 cells Glioblastoma Multiforme Cells Glioblastoma multiforme Hes 1 expressions ic 2015-03-12 03:16:38 Figure https://plos.figshare.com/articles/figure/_The_expression_of_stemness_genes_in_GBM8401_parental_SP_and_non_SP_NSP_cells_/1333970 <p>(A) RT-PCR analysis for the expressions of stemness markers (<i>CD113</i>, <i>Oct-4</i>), neuronal differentiation markers (<i>nestin</i>, <i>β-tubulin III</i>), DNA-repair enzyme (<i>MGMT</i>), stemness signaling pathway (<i>Notch3</i>, <i>IHH</i>, <i>SMO</i>) and drug-resistance genes (<i>ABCG2</i>, <i>MDR1</i>, <i>MGMT</i>). Except the neuronal differentiation marker <i>β-tubulin III</i>, all of the stemness genes expressions in SP cells were significantly higher than those in parental and non-SP (NSP) cells. The <i>β-Actin</i> and <i>GAPDH</i> were used as loading control. (B) Quantitative and statistical analysis data of (A) and two independent similar experiments. *, p<0.05 as compared with parental cells. (C) Western blot analysis for the protein levels of GBM CSC marker CD133 and TMZ resistance-associated protein MGMT. Both CD133 and MGMT protein levels were obviously much higher in the SP cells compared with the parental and non-SP cells. The Tubulin was used as loading control.</p>