%0 Generic %A L. Frank, Kristi %A Colomer-Winter, Cristina %A M. Grindle, Suzanne %A A. Lemos, José %A Schlievert, Patrick M. %A Dunny, Gary M. %D 2014 %T Transcriptome Analysis of Enterococcus faecalis during Mammalian Infection Shows Cells Undergo Adaptation and Exist in a Stringent Response State %U https://plos.figshare.com/articles/dataset/Transcriptome_Analysis_of_Enterococcus_faecalis_during_Mammalian_Infection_Shows_Cells_Undergo_Adaptation_and_Exist_in_a_Stringent_Response_State/1323865 %R 10.1371/journal.pone.0115839 %2 https://ndownloader.figshare.com/files/1932897 %2 https://ndownloader.figshare.com/files/1932898 %2 https://ndownloader.figshare.com/files/1932899 %2 https://ndownloader.figshare.com/files/1932900 %2 https://ndownloader.figshare.com/files/1932901 %K Stringent Response State %K faecalis OG 1RF %K subdermal abscess model %K cell surface components %K subdermal chamber inoculation %K Subsequent subdermal abscess infections %K subdermal abscess formation %K faecalis invivo gene expression %K transcriptional %K Enterococcus faecalis %K Mammalian Infection Shows Cells Undergo Adaptation %K response %K rsh %X

As both a commensal and a major cause of healthcare-associated infections in humans, Enterococcus faecalis is a remarkably adaptable organism. We investigated how E. faecalis adapts in a mammalian host as a pathogen by characterizing changes in the transcriptome during infection in a rabbit model of subdermal abscess formation using transcriptional microarrays. The microarray experiments detected 222 and 291 differentially regulated genes in E. faecalis OG1RF at two and eight hours after subdermal chamber inoculation, respectively. The profile of significantly regulated genes at two hours post-inoculation included genes involved in stress response, metabolism, nutrient acquisition, and cell surface components, suggesting genome-wide adaptation to growth in an altered environment. At eight hours post-inoculation, 88% of the differentially expressed genes were down-regulated and matched a transcriptional profile consistent with a (p)ppGpp-mediated stringent response. Subsequent subdermal abscess infections with E. faecalis mutants lacking the (p)ppGpp synthetase/hydrolase RSH, the small synthetase RelQ, or both enzymes, suggest that intracellular (p)ppGpp levels, but not stringent response activation, influence persistence in the model. The ability of cells to synthesize (p)ppGpp was also found to be important for growth in human serum and whole blood. The data presented in this report provide the first genome-wide insights on E. faecalis invivo gene expression and regulation measured by transcriptional profiling during infection in a mammalian host and show that (p)ppGpp levels affect viability of E. faecalis in multiple conditions relevant to mammalian infection. The subdermal abscess model can serve as a novel experimental system for studying the E. faecalis stringent response in the context of the mammalian immune system.

%I PLOS ONE