%0 Figure %A Iversen, Hildegunn %A Lindbäck, Toril %A M. L’Abée-Lund, Trine %A Roos, Norbert %A Aspholm, Marina %A Stenfors Arnesen, Lotte %D 2015 %T The effect of co-culture on Stx2 production and release of bacteriophages. %U https://plos.figshare.com/articles/figure/_The_effect_of_co_culture_on_Stx2_production_and_release_of_bacteriophages_/1319058 %R 10.1371/journal.pone.0118140.g005 %2 https://ndownloader.figshare.com/files/1925822 %K ttss %K CCUG %K ehec %K Gut Bacterium Bacteroides thetaiotaomicron Influences %K GI tract environment %K stx 2 genes %K lee %K Such interspecies interactions %K secretion system %K NIPH %K gene expression profile %X

(A) Stx2 production by EHEC NIPH-11060424 was measured by reverse passive latex agglutination (RPLA) test. EHEC NIPH-11060424 was co-cultured with B. thetaiotaomicron, using different initial bacterial concentrations. The reciprocal of the highest twofold serial dilution causing latex agglutination was recorded as the titre. Data represent means ± standard errors of the mean (SEM) from three independent experiments. (B) Plaque assay demonstrating production of fewer bacteriophages when EHEC NIPH-11060424 was co-cultured with B. thetaiotaomicron compared to pure culture.

%I PLOS ONE