Anti-metastatic effect of deoxyelephantopin from <i>Elephantopus scaber</i> in A549 lung cancer cells <i>in vitro</i> A.K. Farha S.R. Dhanya S. Nair Mangalam P. Remani 10.6084/m9.figshare.1310474.v2 https://tandf.figshare.com/articles/journal_contribution/Anti_metastatic_effect_of_deoxyelephantopin_from_i_Elephantopus_scaber_i_in_A549_lung_cancer_cells_i_in_vitro_i_/1310474 <div><p>In this study, we focused on the <i>in vitro</i> anti-metastatic effects of deoxyelephantopin (DOE), a sesquiterpene lactone from <i>Elephantopus scaber</i> on lung cancer A549 cells. DOE significantly decreased the metastatic potential of A549 cells as demonstrated by transwell invasion and migration assay. DOE inhibited the expression of matrix metalloproteinase-2 (MMP-2), MMP-9, urokinase-type plasminogen activator and urokinase-type plasminogen activator receptor at transcript level. Tissue inhibitors of metalloproteinase-2 (TIMP-2) mRNA levels was up-regulated in A549 tumour cells without any change in TIMP-1 expression after DOE treatment. DOE inhibited the protein levels of p-ERK1/2 and p-Akt in A549 cells but it activated p-JNK, p-p38 protein expression. NF-κB and IκBα expressions were down-regulated in DOE-treated cells. All these results demonstrated that DOE has shown anti-metastatic activity against A549 tumour cells.</p></div> 2015-10-08 08:37:11 doe 549 tumour cells timp nf 549 lung cancer cells Elephantopus scaber mmp 549 cells expression