10.6084/m9.figshare.1310468.v4
Keita Nishiyama
Keita
Nishiyama
Koichi Nakamata
Koichi
Nakamata
Shintaro Ueno
Shintaro
Ueno
Akari Terao
Akari
Terao
Ni Putu Desy Aryantini
Ni
Putu Desy Aryantini
I. Nengah Sujaya
I.
Nengah Sujaya
Kenji Fukuda
Kenji
Fukuda
Tadasu Urashima
Tadasu
Urashima
Yuji Yamamoto
Yuji
Yamamoto
Takao Mukai
Takao
Mukai
Adhesion properties of <i>Lactobacillus rhamnosus</i> mucus-binding factor to mucin and extracellular matrix proteins
Taylor & Francis Group
2015
overlay dot blot
rhamnosus host colonization
Extracellular matrix proteins
ECM proteins
probiotic Lactobacillus rhamnosus strains
Sumbawa mare milk
mucin
gg
iv
ECM protein binding
FSMM 22 strain
FSMM 22 mbf
pcm
mbf
submicromolar dissociation constants
2015-10-08 18:28:47
Dataset
https://tandf.figshare.com/articles/dataset/Adhesion_properties_of_i_Lactobacillus_rhamnosus_i_mucus_binding_factor_to_mucin_and_extracellular_matrix_proteins/1310468
<div><p>We previously described potential probiotic <i>Lactobacillus rhamnosus</i> strains, isolated from fermented mare milk produced in Sumbawa Island, Indonesia, which showed high adhesion to porcine colonic mucin (PCM) and extracellular matrix (ECM) proteins. Recently, mucus-binding factor (MBF) was found in the GG strain of <i>L. rhamnosus</i> as a mucin-binding protein. In this study, we assessed the ability of recombinant MBF protein from the FSMM22 strain, one of the isolates of <i>L. rhamnosus</i> from fermented Sumbawa mare milk, to adhere to PCM and ECM proteins by overlay dot blot and Biacore assays. MBF bound to PCM, laminin, collagen IV, and fibronectin with submicromolar dissociation constants. Adhesion of the FSMM22 <i>mbf</i> mutant strain to PCM and ECM proteins was significantly less than that of the wild-type strain. Collectively, these results suggested that MBF contribute to <i>L. rhamnosus</i> host colonization via mucin and ECM protein binding.</p></div>