10.6084/m9.figshare.1300047.v2 Madhukar S. Said Madhukar S. Said Ashish A. Chinchansure Ashish A. Chinchansure Laxman Nawale Laxman Nawale Ankita Durge Ankita Durge Ashish Wadhwani Ashish Wadhwani Smita S. Kulkarni Smita S. Kulkarni Dhiman Sarkar Dhiman Sarkar Swati P. Joshi Swati P. Joshi A new butenolide cinnamate and other biological active chemical constituents from <i>Polygonum glabrum</i> Taylor & Francis Group 2015 thp phase Polygonum glabrum Phytochemical investigation pinocembrin subtype compound IC 50 values sitosterol 3.33 IC 50 Mycobacterium tuberculosis H 37Ra HIV MCF 7 cell lines ti 2015-10-08 16:04:43 Journal contribution https://tandf.figshare.com/articles/journal_contribution/A_new_butenolide_cinnamate_and_other_biological_active_chemical_constituents_from_i_Polygonum_glabrum_i_/1300047 <div><p>Phytochemical investigation of the methanol extract of the aerial parts of <i>Polygonum glabrum</i> afforded one new natural product ( − )-2-methoxy-2-butenolide-3-cinnamate (<b>1</b>) along with six known compounds, β-hydroxyfriedalanol (<b>2</b>), 3-hydroxy-5-methoxystilbene (<b>3</b>), ( − ) pinocembrin (<b>4</b>), sitosterol-(6′-<i>O</i>-palmitoyl)-3-<i>O</i>-β-d-glucopyranoside (<b>5</b>), ( − ) pinocembrin-5-methyl ether (<b>6</b>) and sitosterol-3-<i>O</i>-β-d-glucopyranoside (<b>7</b>). Compound <b>1</b> showed promising <i>in vitro</i> anti-HIV-1 activity against primary isolates HIV-1<sub>UG070</sub> (X4, subtype D) and HIV-1<sub>VB59</sub> (R5, subtype C) assayed using TZM-bl cell line with IC<sub>50</sub> in the range of 15.68–22.43 μg/mL. The extract showed TI in the range of 19.19–27.37 with IC<sub>50</sub> in the range of 10.90–15.55 μg/mL. Compounds <b>1</b>, <b>3</b> and <b>4</b> exhibited <i>in vitro</i> anti-mycobacterium activity against <i>Mycobacterium tuberculosis</i> H37Ra with IC<sub>50</sub> values of 1.43, 3.33 and 1.11 μg/mL in dormant phase and 2.27, 3.33 and 1.21 μg/mL in active phase, respectively. Compound <b>4</b> was found to be the most active antiproliferative with IC<sub>50</sub> values of 1.88–11.00 μg/mL against THP-1, A549, Panc-1, HeLa and MCF7 cell lines.</p></div>