Ohkama-Ohtsu, Naoko Ichida, Sachiko Yamaya, Hiroko Ohwada, Takuji Itakura, Manabu Hara, Yoshino Mitsui, Hisayuki Kaneko, Takakazu Tabata, Satoshi Tejima, Kouhei Saeki, Kazuhiko Omori, Hirofumi Hayashi, Makoto Maekawa, Takaki Murooka, Yoshikatsu Tajima, Shigeyuki Simomura, Kenshiro Nomura, Mika Uchiumi, Toshiki Suzuki, Akihiro Shimoda, Yoshikazu Abe, Mikiko Minamisawa, Kiwamu Arima, Yasuhiro Yokoyama, Tadashi Peribacteroid solution of soybean root nodules partly induces genomic loci for differentiation into bacteroids of free-living <i>Bradyrhizobium japonicum</i> cells <p>In leguminous root nodules, rhizobia differentiate into morphology specific to symbiosis, called bacteroids. As bacteroids are surrounded with peribacteroid membranes filled with peribacteroid solution (PBS), it is considered that PBS contains substances inducing differentiation of rhizobia into bacteroids. In this study, genome-wide expression profiles of <i>Bradyrhizobium japonicum</i> cells cultured in PBS purified from root nodule of soybean (<i>Glycine max</i> L.) were compared with those of bacteroids using macroarray. PBS treatment preferentially induced regions in a large symbiosis island including various symbiosis relevant genes such as <i>nod, fix, nol</i> and <i>noe</i>, in which 75% of regions were commonly induced in bacteroids, while general repressions outside of the symbiosis island seen in bacteroids were not observed in PBS treated cells. The present results suggest that PBS contained some, but not all, substances inducing expression of the genes which are involved in differentiation into bacteroids.</p> bacteroid;Bradyrhizobium japonicum;macroarray;peribacteroid solution;soybean 2015-06-26
    https://tandf.figshare.com/articles/dataset/Peribacteroid_solution_of_soybean_root_nodules_partly_induces_genomic_loci_for_differentiation_into_bacteroids_of_free_living_i_Bradyrhizobium_japonicum_i_cells/1287664
10.6084/m9.figshare.1287664.v2