10.1371/journal.pone.0115684.g005 Shoichiro Tange Shoichiro Tange Dulamsuren Oktyabri Dulamsuren Oktyabri Minoru Terashima Minoru Terashima Akihiko Ishimura Akihiko Ishimura Takeshi Suzuki Takeshi Suzuki Knockdown of <i>JARID2</i> affected the TGF-ß-dependent changes in expression of EMT-related genes in A549 cells. Public Library of Science 2014 Colon Cancer Cell Lines Histone methylation EZH 2 occupancies tgf PRC 2 recruitment 3k emt Colon cancer cell lines gene expression JARID 2 JARID 2 protein JARID 2 knockdown zeb Chromatin immunoprecipitation assays cdh 549 lung cancer cell line histone H 3 methylation HT 29 colon cancer cell line histone H 3 2014-12-26 03:21:04 Figure https://plos.figshare.com/articles/figure/_Knockdown_of_JARID2_affected_the_TGF_dependent_changes_in_expression_of_EMT_related_genes_in_A549_cells_/1280178 <p>QRT-PCR analysis was performed to detect the expression of <i>CDH1/E-cadherin</i> (A), <i>FN1/Fibronectin</i> (B), <i>Vimentin</i> (C), <i>ZEB1</i> (D), <i>ZEB2</i> (E), <i>miR-200a</i> (F), <i>miR-200c</i> (G) and <i>JARID1B</i> (H) in A549 cells infected with retroviruses expressing control shRNA or <i>JARID2</i> shRNA with or without the treatment of 1 ng/ml of TGF-ß for 24 hours (*, <i>P</i><0.01 comparing to control). (I) Western blot was performed to detect the expression of E-cadherin, Fibronectin, Vimentin, ZEB1, ZEB2, phosphorylated SMAD3 (P-SMAD3) and GAPDH proteins using the corresponding antibodies.</p>