Sumru Bayin, N. S. Modrek, Aram Dietrich, August Lebowitz, Jonathan Abel, Tobias Song, Hae-Ri Schober, Markus Zagzag, David J. Buchholz, Christian Chao, Moses V. G. Placantonakis, Dimitris CD133-LV does not transduce normal mouse brain cells, hESC-derived neurons and primary human astrocytes <i>in vitro</i>. <p><b>A</b>. Injection of CD133-LV expressing mCherry into the mouse basal ganglia did not lead to transduction of normal brain tissue, as opposed to VSVG-LV (BG: basal ganglia, Cx: cortex, CC: corpus callosum, LV: lateral ventricle). <b>Bi,ii</b>. hESC-derived neurons were transduced with either CD133-LV or VSVG-LV expressing mCherry. VSVG-LV led to transduction of MAP2A+ neurons, as opposed to CD133-LV. <b>Ci,ii</b>. Primary human astrocytes were transduced with either CD133-LV or VSVG-LV expressing mCherry (MOI = 10). VSVG-LV led to transduction of GFAP+ astrocytes, as opposed to CD133-LV. (NeuN: neural nuclei, MAP2A: microtubule associated protein 2A, GFAP: glial fibrillary acidic protein). Nuclei were counterstained with DAPI.</p> gsc;glycosylated pentaspan transmembrane protein;gbm;CD 133;cause tumor recurrence;Selective Lentiviral Gene Delivery;NOD.SCID;mouse brain tissue;cell surface expression 2014-12-26
    https://plos.figshare.com/articles/figure/_CD133_LV_does_not_transduce_normal_mouse_brain_cells_hESC_derived_neurons_and_primary_human_astrocytes_in_vitro_/1280176
10.1371/journal.pone.0116114.g005