10.1371/journal.pone.0115920.g003 Anita Posevitz-Fejfár Anita Posevitz-Fejfár Vilmos Posevitz Vilmos Posevitz Catharina C. Gross Catharina C. Gross Urvashi Bhatia Urvashi Bhatia Frank Kurth Frank Kurth Verena Schütte Verena Schütte Amit Bar-Or Amit Bar-Or Sven G. Meuth Sven G. Meuth Heinz Wiendl Heinz Wiendl The effects of overnight shipping on T cell proliferation. Public Library of Science 2014 proliferative capacity cell subsets B cell frequencies Various control experiments Human Peripheral Mononuclear Cell Yield Immune Cell Biobanking Human biospecimen collection il pbmc nk dna blood transportation 2014-12-26 03:21:12 Figure https://plos.figshare.com/articles/figure/_The_effects_of_overnight_shipping_on_T_cell_proliferation_/1280174 <p>PBMC from non-shipped vs. shipped samples were thawed, loaded with eFluor670 cell proliferation dye and stimulated with plate bound anti-CD3 (OKT3) and soluble anti-CD28 antibodies for 6 days (as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115920#s2" target="_blank">methods</a>). Cells were harvested and proliferation was analyzed by flow cytometry. A) A representative graph is shown for CD8<sup>+</sup> and CD4<sup>+</sup> T cell proliferation. B) Bar graphs summarize the percentage of proliferating CD8<sup>+</sup> (left) and CD4<sup>+</sup> (right) T cells of all donors (n = 9).</p>