10.1371/journal.pone.0115920.g003
Anita Posevitz-Fejfár
Anita
Posevitz-Fejfár
Vilmos Posevitz
Vilmos
Posevitz
Catharina C. Gross
Catharina
C. Gross
Urvashi Bhatia
Urvashi
Bhatia
Frank Kurth
Frank
Kurth
Verena Schütte
Verena
Schütte
Amit Bar-Or
Amit
Bar-Or
Sven G. Meuth
Sven
G. Meuth
Heinz Wiendl
Heinz
Wiendl
The effects of overnight shipping on T cell proliferation.
Public Library of Science
2014
proliferative capacity
cell subsets
B cell frequencies
Various control experiments
Human Peripheral Mononuclear Cell Yield
Immune Cell Biobanking Human biospecimen collection
il
pbmc
nk
dna
blood transportation
2014-12-26 03:21:12
Figure
https://plos.figshare.com/articles/figure/_The_effects_of_overnight_shipping_on_T_cell_proliferation_/1280174
<p>PBMC from non-shipped vs. shipped samples were thawed, loaded with eFluor670 cell proliferation dye and stimulated with plate bound anti-CD3 (OKT3) and soluble anti-CD28 antibodies for 6 days (as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115920#s2" target="_blank">methods</a>). Cells were harvested and proliferation was analyzed by flow cytometry. A) A representative graph is shown for CD8<sup>+</sup> and CD4<sup>+</sup> T cell proliferation. B) Bar graphs summarize the percentage of proliferating CD8<sup>+</sup> (left) and CD4<sup>+</sup> (right) T cells of all donors (n = 9).</p>