10.1371/journal.pone.0115949.g004 Ning Zhang Ning Zhang Lu Wang Lu Wang Zong-Tao Chai Zong-Tao Chai Zi-Man Zhu Zi-Man Zhu Xiao-Dong Zhu Xiao-Dong Zhu De-Ning Ma De-Ning Ma Qiang-Bo Zhang Qiang-Bo Zhang Yi-Ming Zhao Yi-Ming Zhao Miao Wang Miao Wang Jian-Yang Ao Jian-Yang Ao Zheng-Gang Ren Zheng-Gang Ren Dong-Mei Gao Dong-Mei Gao Hui-Chuan Sun Hui-Chuan Sun Zhao-You Tang Zhao-You Tang Residual tumors after incomplete RFA exhibited changes consistent with EMT. Public Library of Science 2014 HCC cell RFA orthotopic HCC Residual Cancer EMT phenotype changes vivo study HCCLM 3 cells Mouse models metastatic HCC cell line HCCLM 3 RFA orthotopic heat intervention Hepatocellular Carcinoma Cell HCCLM 3 Hepatocellular carcinoma Incomplete Radiofrequency Ablation Enhances Invasiveness Mouse Model catenin RFA intervention HCC cells vitro.ConclusionIncomplete RFA intraperitoneal metastasis 2014-12-26 03:20:26 Figure https://plos.figshare.com/articles/figure/_Residual_tumors_after_incomplete_RFA_exhibited_changes_consistent_with_EMT_/1280172 <p>(A) Immunohistochemistry revealed that E-cadherin expression was decreased in incomplete RFA group of both HCC cell models, the expression level of N-cadherin in tumor tissues were higher in incomplete RFA group than that in controls, and in HCCLM3-G model, increased expression of vimentin was also detected in incomplete RFA group. (B) Western blot showed the changes of residual tumors were consistent with EMT in incomplete RFA group (e.g., down-regulation of E-cadherin and up-regulation of N-cadherin, vimentin) of both HCC cell models. (C) RT-PCR showed the expression of transcription factors Snail, Slug, and Twist at the mRNA level in tumor tissue. However, only Snail mRNAs in HCCLM3-G model and Slug mRNA in HepG2-G model were significantly up-regulated in incomplete RFA group, respectively (<i>P</i><0.05).</p>