CD133-LV transduces CD133+ cells in primary GBM xenografts in the mouse brain.
N. Sumru Bayin
Aram S. Modrek
August Dietrich
Jonathan Lebowitz
Tobias Abel
Hae-Ri Song
Markus Schober
David Zagzag
Christian J. Buchholz
Moses V. Chao
Dimitris G. Placantonakis
10.1371/journal.pone.0116114.g004
https://plos.figshare.com/articles/figure/_CD133_LV_transduces_CD133_cells_in_primary_GBM_xenografts_in_the_mouse_brain_/1280171
<p><b>A</b>. Intracranial xenograft tumors were generated using injection of GBML20 cells (5×10<sup>5</sup> cells/animal) and tumor formation (red circle) was confirmed with small animal MRI 1.5 months after injection. High titer stocks of CD133-LV or VSVG-LV expressing TagBFP were injected into the tumor and animals were sacrificed 7 days later for immunofluorescence analysis. <b>B</b>. CD133-LV - transduced cells expressing TagBFP (red) show cell surface immunoreactivity for CD133 (green). <b>C</b>. CD133+ cells were significantly more enriched among TagBFP+ transduced cells in the case of CD133-LV compared to VSVG-LV.</p>
2014-12-26 03:21:50
gsc
glycosylated pentaspan transmembrane protein
gbm
CD 133
cause tumor recurrence
Selective Lentiviral Gene Delivery
NOD.SCID
mouse brain tissue
cell surface expression