CD133-LV transduces CD133+ cells in primary GBM xenografts in the mouse brain. N. Sumru Bayin Aram S. Modrek August Dietrich Jonathan Lebowitz Tobias Abel Hae-Ri Song Markus Schober David Zagzag Christian J. Buchholz Moses V. Chao Dimitris G. Placantonakis 10.1371/journal.pone.0116114.g004 https://plos.figshare.com/articles/figure/_CD133_LV_transduces_CD133_cells_in_primary_GBM_xenografts_in_the_mouse_brain_/1280171 <p><b>A</b>. Intracranial xenograft tumors were generated using injection of GBML20 cells (5×10<sup>5</sup> cells/animal) and tumor formation (red circle) was confirmed with small animal MRI 1.5 months after injection. High titer stocks of CD133-LV or VSVG-LV expressing TagBFP were injected into the tumor and animals were sacrificed 7 days later for immunofluorescence analysis. <b>B</b>. CD133-LV - transduced cells expressing TagBFP (red) show cell surface immunoreactivity for CD133 (green). <b>C</b>. CD133+ cells were significantly more enriched among TagBFP+ transduced cells in the case of CD133-LV compared to VSVG-LV.</p> 2014-12-26 03:21:50 gsc glycosylated pentaspan transmembrane protein gbm CD 133 cause tumor recurrence Selective Lentiviral Gene Delivery NOD.SCID mouse brain tissue cell surface expression