Durand, Sylvain Gilet, Laetitia Bessières, Philippe Nicolas, Pierre Condon, Ciarán Three Essential Ribonucleases—RNase Y, J1, and III—Control the Abundance of a Majority of <em>Bacillus subtilis</em> mRNAs <div><p><em>Bacillus subtilis</em> possesses three essential enzymes thought to be involved in mRNA decay to varying degrees, namely RNase Y, RNase J1, and RNase III. Using recently developed high-resolution tiling arrays, we examined the effect of depletion of each of these enzymes on RNA abundance over the whole genome. The data are consistent with a model in which the degradation of a significant number of transcripts is dependent on endonucleolytic cleavage by RNase Y, followed by degradation of the downstream fragment by the 5′–3′ exoribonuclease RNase J1. However, many full-size transcripts also accumulate under conditions of RNase J1 insufficiency, compatible with a model whereby RNase J1 degrades transcripts either directly from the 5′ end or very close to it. Although the abundance of a large number of transcripts was altered by depletion of RNase III, this appears to result primarily from indirect transcriptional effects. Lastly, RNase depletion led to the stabilization of many low-abundance potential regulatory RNAs, both in intergenic regions and in the antisense orientation to known transcripts.</p> </div> abundance;mrnas 2012-03-08
    https://plos.figshare.com/articles/dataset/Three_Essential_Ribonucleases_RNase_Y_J1_and_III_Control_the_Abundance_of_a_Majority_of_Bacillus_subtilis_mRNAs/128013
10.1371/journal.pgen.1002520