10.1371/journal.pgen.1002520 Sylvain Durand Sylvain Durand Laetitia Gilet Laetitia Gilet Philippe Bessières Philippe Bessières Pierre Nicolas Pierre Nicolas Ciarán Condon Ciarán Condon Three Essential Ribonucleases—RNase Y, J1, and III—Control the Abundance of a Majority of <em>Bacillus subtilis</em> mRNAs Public Library of Science 2012 abundance mrnas 2012-03-08 02:13:33 Dataset https://plos.figshare.com/articles/dataset/Three_Essential_Ribonucleases_RNase_Y_J1_and_III_Control_the_Abundance_of_a_Majority_of_Bacillus_subtilis_mRNAs/128013 <div><p><em>Bacillus subtilis</em> possesses three essential enzymes thought to be involved in mRNA decay to varying degrees, namely RNase Y, RNase J1, and RNase III. Using recently developed high-resolution tiling arrays, we examined the effect of depletion of each of these enzymes on RNA abundance over the whole genome. The data are consistent with a model in which the degradation of a significant number of transcripts is dependent on endonucleolytic cleavage by RNase Y, followed by degradation of the downstream fragment by the 5′–3′ exoribonuclease RNase J1. However, many full-size transcripts also accumulate under conditions of RNase J1 insufficiency, compatible with a model whereby RNase J1 degrades transcripts either directly from the 5′ end or very close to it. Although the abundance of a large number of transcripts was altered by depletion of RNase III, this appears to result primarily from indirect transcriptional effects. Lastly, RNase depletion led to the stabilization of many low-abundance potential regulatory RNAs, both in intergenic regions and in the antisense orientation to known transcripts.</p> </div>