TY - DATA T1 - Comparative proteomic analysis of Arabidopsis thaliana roots between wild type and its salt-tolerant mutant PY - 2014/01/02 AU - Meili Guo AU - Hua Li AU - Lei Li AU - Weixi Gao AU - Yulan Xu AU - Chunxi Zhou AU - Xin Liu AU - Xuemei Cheng AU - Fujun Liu UR - https://tandf.figshare.com/articles/journal_contribution/Comparative_proteomic_analysis_of_i_Arabidopsis_thaliana_i_roots_between_wild_type_and_its_salt_tolerant_mutant/1278879 DO - 10.6084/m9.figshare.1278879.v1 L4 - https://ndownloader.figshare.com/files/1854509 KW - redox homeostasis KW - Comparative Proteomic Analysis KW - ms KW - flight mass spectrometry KW - cell wall metabolism KW - de KW - Arabidopsis thaliana root proteins KW - Arabidopsis thaliana roots KW - 14 protein spots KW - root protein spots N2 - Two-dimensional electrophoresis (2-DE) showed the variation expression of Arabidopsis thaliana root proteins between wild type and its salt-tolerant mutant obtained from cobalt-60 γ ray radiation. Forty-six differential root protein spots were reproducibly presented on 2-DE maps, and 29 spots were identified by matrix assisted laser desorption ionization-time of flight/time of flight mass spectrometry (MS). Fifteen protein spots corresponding to 10 proteins, and 14 protein spots corresponding to 9 proteins were constitutively up-regulated and down-regulated in the salt-tolerant mutant root. Bioinformatic analysis indicated that those differential proteins might be involved in the regulation of redox homeostasis, nucleotide metabolism, signal transduction, stress response and defense, carbohydrate metabolism, and cell wall metabolism. Peroxidase 22 might be a versatile enzyme and might play dual roles in both cell wall metabolism and regulation of redox homeostasis. Our work provides not only new insights into salt-responsive proteins in root, but also the potential salt-tolerant targets for further dissection of molecular mechanism adapted by plants during salt stress. ER -