10.1371/journal.pone.0114691.g005 Kristof Moonens Kristof Moonens Maia De Kerpel Maia De Kerpel Annelies Coddens Annelies Coddens Eric Cox Eric Cox Els Pardon Els Pardon Han Remaut Han Remaut Henri De Greve Henri De Greve Conformational change induced inhibition of adhesion by nanobodies NbFedF6 and NbFedF7. Public Library of Science 2014 Protective treatments farming industry attachment Nanobody Mediated Inhibition FedF surface fimbriated pathogens piglet enterocytes blood group antigen receptors membrane context nanobodie lectin domain adhesin domain F 18 fimbrial adhesin FedF FedF lectin domain F 18 fimbriated E coli bacteria CDR 3 region F 18 fimbriated bacteria adherence assay binding site blood group antigen receptor F 18 Fimbriae edema disease production losses loop 2014-12-11 03:04:10 Figure https://plos.figshare.com/articles/figure/_Conformational_change_induced_inhibition_of_adhesion_by_nanobodies_NbFedF6_and_NbFedF7_/1268302 <p>Superimposition of the FedF-blood group A type 1 hexasaccharide (A6-1) co-complex structure (colored yellow) with the co-complex structures of FedF-NbFedF6 (<b>A</b>) and FedF-NbFedF7 (<b>B</b>) (β-strands, α-helices and loops are colored respectively cyan, red and grey). Both NbFedF6 and NbFedF7 induce a conformational change in the D″-E loop that is protruding from the FedF surface thereby reorienting the loop outwards from the A6-1 binding site. A6-1 is depicted in stick model with carbon, oxygen and nitrogen atoms colored respectively purple, red and blue.</p>